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Sample GSM465895 Query DataSets for GSM465895
Status Public on Oct 01, 2014
Title midgut non-infected control rep-a
Sample type RNA
 
Source name midgut non-infected control rep-a
Organism Anopheles gambiae
Characteristics tissue: midgut
infection: control
Growth protocol Anopheles gambiae mosquitoes were infected with Plasmodium falciparum (3D7) by allowing the mosquitoes to feed on infected mice. The dissected tissues were immediately placed on RNAlater solution (Ambion Inc.) and stored at -80C until further processing.
Extracted molecule total RNA
Extraction protocol Three pools, each one containing whole organs from approximately 20 mosquitos were used for total RNA extraction using Trizol reagent (Invitrogen). For the infected organs we extracted the mosquito and parasite RNA simultaneously.
Label biotin
Label protocol RNA was amplified and labeled using two-rounds of linear amplification according to the manufacturer’s protocol (Affymetrix GeneChip® Two-Cycle cDNA Synthesis Kit)
 
Hybridization protocol Hybridized at a constant temperature of 45◦C for approximately forty-eight hours using the Affymetrix 640 oven 500k approved
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 7GPlus.
Description Gene expression data from_midgut non-infected control rep-a
Data processing The data were analyzed with GCOS 1.4 using Affymetrix default analysis with a scale filter for Ft genes setting and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
 
Submission date Oct 28, 2009
Last update date Oct 01, 2014
Contact name Dan Sturdevant
E-mail(s) dsturdevant@niaid.nih.gov
Phone 4063639248
Organization name NIH
Department NIAID
Lab RTS
Street address 903 S 4th street
City Hamilton
State/province MT
ZIP/Postal code 59840
Country USA
 
Platform ID GPL1321
Series (1)
GSE18780 Gene profiling of P. falciparum and A. gambiae: an assessment of parasite maturation and of host response to infection

Data table header descriptions
ID_REF
VALUE Signal
ABS_CALL indicating whether the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 7692.53 P 0.0166701
AFFX-BioB-M_at 8266.97 P 0.0012475
AFFX-BioB-3_at 5715.63 P 0.00687065
AFFX-BioC-5_at 24218.2 P 0.00010954
AFFX-BioC-3_at 24875.8 P 4.42873e-05
AFFX-BioDn-5_at 42169.9 P 4.42873e-05
AFFX-BioDn-3_at 114588 P 0.00010954
AFFX-CreX-5_at 273139 P 4.42873e-05
AFFX-CreX-3_at 451140 P 4.42873e-05
AFFX-DapX-5_at 8313.66 P 0.000126798
AFFX-DapX-M_at 52353.1 P 0.000753643
AFFX-DapX-3_at 151116 P 4.42873e-05
AFFX-LysX-5_at 1227.49 A 0.165861
AFFX-LysX-M_at 8900.29 P 0.0284573
AFFX-LysX-3_at 38538 P 5.16732e-05
AFFX-PheX-5_at 2924.27 A 0.083592
AFFX-PheX-M_at 5681.75 P 0.0166701
AFFX-PheX-3_at 23298.7 P 4.42873e-05
AFFX-ThrX-5_at 1583.82 A 0.425962
AFFX-ThrX-M_at 12321.3 P 0.00556451

Total number of rows: 22769

Table truncated, full table size 856 Kbytes.




Supplementary file Size Download File type/resource
GSM465895.CEL.gz 2.3 Mb (ftp)(http) CEL
GSM465895.CHP.gz 122.0 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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