|
| Status |
Public on Oct 01, 2014 |
| Title |
midgut infected rep-a |
| Sample type |
RNA |
| |
|
| Source name |
midgut infected rep-a
|
| Organisms |
Plasmodium falciparum; Anopheles gambiae |
| Characteristics |
tissue: midgut
infection: infected with P. falciparum
|
| Growth protocol |
Anopheles gambiae mosquitoes were infected with Plasmodium falciparum (3D7) by allowing the mosquitoes to feed on infected mice. The dissected tissues were immediately placed on RNAlater solution (Ambion Inc.) and stored at -80C until further processing.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Three pools, each one containing whole organs from approximately 20 mosquitos were used for total RNA extraction using Trizol reagent (Invitrogen). For the infected organs we extracted the mosquito and parasite RNA simultaneously.
|
| Label |
biotin
|
| Label protocol |
RNA was amplified and labeled using two-rounds of linear amplification according to the manufacturer’s protocol (Affymetrix GeneChip® Two-Cycle cDNA Synthesis Kit)
|
| |
|
| Hybridization protocol |
Hybridized at a constant temperature of 45◦C for approximately forty-eight hours using the Affymetrix 640 oven 500k approved
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 7GPlus.
|
| Description |
Gene expression data from_midgut infected rep-a
|
| Data processing |
The data were analyzed with GCOS 1.4 using Affymetrix default analysis with a scale filter for Ft genes setting and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
|
| |
|
| Submission date |
Oct 28, 2009 |
| Last update date |
Oct 01, 2014 |
| Contact name |
Dan Sturdevant |
| E-mail(s) |
dsturdevant@niaid.nih.gov
|
| Phone |
4063639248
|
| Organization name |
NIH
|
| Department |
NIAID
|
| Lab |
RTS
|
| Street address |
903 S 4th street
|
| City |
Hamilton |
| State/province |
MT |
| ZIP/Postal code |
59840 |
| Country |
USA |
| |
|
| Platform ID |
GPL1321 |
| Series (1) |
| GSE18780 |
Gene profiling of P. falciparum and A. gambiae: an assessment of parasite maturation and of host response to infection |
|
