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Status |
Public on Oct 30, 2009 |
Title |
Embryo_Rad21 mutant_48hpf_biological rep 1 |
Sample type |
RNA |
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Source name |
Embryo_Rad21 mutant_48hpf
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Organism |
Danio rerio |
Characteristics |
tissue: Dechorionated embryo genotype/variation: Rad21 mutant time point: 48 hours post-fertilization
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Treatment protocol |
Rad21nz171 homozgous mutant embryos were compared to wild type embryos at the same developmental time.
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Growth protocol |
Embryos were kept in embryo medium at 28 degrees celcius until collected for RNA extraction.
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Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using Trizol (Invitrogen) and purfied using Qiagen Rneasy columns.
|
Label |
biotin
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Label protocol |
Total RNA was labelled using Affymetrix GeneChip® One-Cycle Target Labeling and Control Reagents Kit Cat # 900493 using standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual, rev.3. 2001)
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Hybridization protocol |
Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual, rev.3. 2001) using Affymetrix GeneChip® Hybrisiation Oven 640
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Scan protocol |
Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual, rev.3. 2001) using Affymetrix GeneChip® Scanner 7G
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Description |
Gene Expressiondata from Rad21 mutants at 48hpf
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Data processing |
After QC using GCOS and dCHhip, probe level data were background corrected, normalised and summarised using the default RMA method (PM only) in the Affy package of the R statistical environment.
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Submission date |
Oct 29, 2009 |
Last update date |
Oct 29, 2009 |
Contact name |
Julia Horsfield |
E-mail(s) |
julia.horsfield@otago.ac.nz
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Organization name |
University of Otago
|
Department |
Pathology Department
|
Lab |
Chromosome Structure and Development Group
|
Street address |
Cnr Hanover and Great King Streets
|
City |
Dunedin |
ZIP/Postal code |
9016 |
Country |
New Zealand |
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Platform ID |
GPL1319 |
Series (1) |
GSE18795 |
Expression data from zebrafish embryos homozygous mutant for the cohesin subunit Rad21 |
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