|
| Status |
Public on Oct 30, 2009 |
| Title |
Embryo_Rad21 mutant_48hpf_biological rep 4 |
| Sample type |
RNA |
| |
|
| Source name |
Embryo_Rad21 mutant_48hpf
|
| Organism |
Danio rerio |
| Characteristics |
tissue: Dechorionated embryo
genotype/variation: Rad21 mutant
time point: 48 hours post-fertilization
|
| Treatment protocol |
Rad21nz171 homozgous mutant embryos were compared to wild type embryos at the same developmental time.
|
| Growth protocol |
Embryos were kept in embryo medium at 28 degrees celcius until collected for RNA extraction.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Trizol (Invitrogen) and purfied using Qiagen Rneasy columns.
|
| Label |
biotin
|
| Label protocol |
Total RNA was labelled using Affymetrix GeneChip® One-Cycle Target Labeling and Control Reagents Kit Cat # 900493 using standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual, rev.3. 2001)
|
| |
|
| Hybridization protocol |
Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual, rev.3. 2001) using Affymetrix GeneChip® Hybrisiation Oven 640
|
| Scan protocol |
Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual, rev.3. 2001) using Affymetrix GeneChip® Scanner 7G
|
| Description |
Gene Expressiondata from Rad21 mutants at 48hpf
|
| Data processing |
After QC using GCOS and dCHhip, probe level data were background corrected, normalised and summarised using the default RMA method (PM only) in the Affy package of the R statistical environment.
|
| |
|
| Submission date |
Oct 29, 2009 |
| Last update date |
Oct 29, 2009 |
| Contact name |
Julia Horsfield |
| E-mail(s) |
julia.horsfield@otago.ac.nz
|
| Organization name |
University of Otago
|
| Department |
Pathology Department
|
| Lab |
Chromosome Structure and Development Group
|
| Street address |
Cnr Hanover and Great King Streets
|
| City |
Dunedin |
| ZIP/Postal code |
9016 |
| Country |
New Zealand |
| |
|
| Platform ID |
GPL1319 |
| Series (1) |
| GSE18795 |
Expression data from zebrafish embryos homozygous mutant for the cohesin subunit Rad21 |
|
