|
Status |
Public on Oct 30, 2009 |
Title |
ASD61A_Exo_1 |
Sample type |
RNA |
|
|
Source name |
Exosomes produced by AS-Tspan8 cells
|
Organism |
Rattus norvegicus |
Characteristics |
strain: BDX tissue: pancreas cells/exosomes: exosomes
|
Treatment protocol |
Prior RNA isolation, endothelial cells were starved overnight, then RPMI medium supplemented with 2% FCS with or without 10 ug/ml exosomes or VEGF was added and incubated for 24 hours.
|
Growth protocol |
Tumour cells were cultured in RPMI medium supplemented with 10% FCS and 2mM Gluthamined. For exosomes isolation cells were starved for 36 hours, and exosomes were isolated from serum-free supernatants. Endothelial cells were maintananed in EBM2 (Promocells) or MCDB131 (Invitrogen) cell culture medium supplented with 2-5% FCS and 5ng/ml VEGF.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
Label |
Cy3
|
Label protocol |
Standard Illumina hybridization protocol, performed in the core facility of german cancer research center (DKFZ), Hedelberg, Germany
|
|
|
Hybridization protocol |
Standard Illumina scanning protocol, performed in the core facility of german cancer research center (DKFZ), Hedelberg, Germany
|
Scan protocol |
The data were proceeced and analysed by the corer facility of german cancer research center (DKFZ), Hedelberg, Germany
|
Description |
SAMPLE 11 replica1
|
Data processing |
The data were proceeced and analysed by the corer facility of german cancer research center (DKFZ), Hedelberg, Germany; the Raw data background (non-related probes) (71 +/-5); system noise (non-probe related signals) (12 +/-4)
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|
|
Submission date |
Oct 29, 2009 |
Last update date |
Oct 29, 2009 |
Contact name |
Irina Nazarenko |
E-mail(s) |
irina.nazarenko@kit.edu
|
Phone |
+49-1793237603
|
Organization name |
Karlsruhe Insitute of Technology (KIT)
|
Department |
Institute of Toxicology and Genetics
|
Street address |
Hermann-von-Helmholtz-Platz 1
|
City |
Eggenstein-Leopoldshafen |
ZIP/Postal code |
76344 |
Country |
Germany |
|
|
Platform ID |
GPL6101 |
Series (1) |
GSE18812 |
Comparative genome analysis of exosomal mRNA derived from Tspan8-overexpressing and Tspan8-negative rat pancreas tumors |
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