|
| Status |
Public on Oct 30, 2009 |
| Title |
ASD61A_Exo_2 |
| Sample type |
RNA |
| |
|
| Source name |
Exosomes produced by AS-Tspan8 cells
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: BDX
tissue: pancreas
cells/exosomes: exosomes
|
| Treatment protocol |
Prior RNA isolation, endothelial cells were starved overnight, then RPMI medium supplemented with 2% FCS with or without 10 ug/ml exosomes or VEGF was added and incubated for 24 hours.
|
| Growth protocol |
Tumour cells were cultured in RPMI medium supplemented with 10% FCS and 2mM Gluthamined. For exosomes isolation cells were starved for 36 hours, and exosomes were isolated from serum-free supernatants. Endothelial cells were maintananed in EBM2 (Promocells) or MCDB131 (Invitrogen) cell culture medium supplented with 2-5% FCS and 5ng/ml VEGF.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
Cy3
|
| Label protocol |
Standard Illumina hybridization protocol, performed in the core facility of german cancer research center (DKFZ), Hedelberg, Germany
|
| |
|
| Hybridization protocol |
Standard Illumina scanning protocol, performed in the core facility of german cancer research center (DKFZ), Hedelberg, Germany
|
| Scan protocol |
The data were proceeced and analysed by the corer facility of german cancer research center (DKFZ), Hedelberg, Germany
|
| Description |
SAMPLE 12
replica2
|
| Data processing |
The data were proceeced and analysed by the corer facility of german cancer research center (DKFZ), Hedelberg, Germany; the Raw data background (non-related probes) (71 +/-5); system noise (non-probe related signals) (12 +/-4)
|
| |
|
| Submission date |
Oct 29, 2009 |
| Last update date |
Oct 29, 2009 |
| Contact name |
Irina Nazarenko |
| E-mail(s) |
irina.nazarenko@kit.edu
|
| Phone |
+49-1793237603
|
| Organization name |
Karlsruhe Insitute of Technology (KIT)
|
| Department |
Institute of Toxicology and Genetics
|
| Street address |
Hermann-von-Helmholtz-Platz 1
|
| City |
Eggenstein-Leopoldshafen |
| ZIP/Postal code |
76344 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6101 |
| Series (1) |
| GSE18812 |
Comparative genome analysis of exosomal mRNA derived from Tspan8-overexpressing and Tspan8-negative rat pancreas tumors |
|
