|
| Status |
Public on Dec 31, 2009 |
| Title |
Zebrafish QKD embryo_ 30%E_biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Zebrafish sox2/3/19a/19b knockdown embryo at 30% epiboly stage
|
| Organism |
Danio rerio |
| Characteristics |
tissue: whole embryo
strain: TL
|
| Growth protocol |
Embryos were grown in in 0.03% Red Sea salt solution.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was prepared from 100 embryos using Ribo Pure kit (Ambion).
|
| Label |
biotin
|
| Label protocol |
cRNA probes were prepared using 4 microgram total RNA with a one-cycle cDNA synthesis kit (Affymetrix).
|
| |
|
| Hybridization protocol |
10 micrograms of fragmented cRNA probes were hybridized to the GeneChip array and posthybridization staining and washing were performed according to the manufacture’s instruction (Affymetrix).
|
| Scan protocol |
GeneChip arrays were scanned with GS3000 scanner.
|
| Description |
Gene expression data from embryos injected with MOs for sox2/3/19a/19b quadruple knockdown at 30% epiboly stage.
|
| Data processing |
Data were processed using the RMA program.
|
| |
|
| Submission date |
Oct 30, 2009 |
| Last update date |
Oct 30, 2009 |
| Contact name |
Yusuke Kamachi |
| E-mail(s) |
yusuke@fbs.osaka-u.ac.jp
|
| Organization name |
Osaka University
|
| Department |
Graduate School of Frontier Biosciences
|
| Street address |
1-3 Yamadaoka
|
| City |
Suita |
| State/province |
Osaka |
| ZIP/Postal code |
565-0871 |
| Country |
Japan |
| |
|
| Platform ID |
GPL1319 |
| Series (1) |
| GSE18830 |
B1 sox (sox2/3/19a/19b) quadruple knockdown in the zebrafish embryo |
|
