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Status |
Public on Dec 31, 2009 |
Title |
Zebrafish QKD embryo_ 75%E_biological rep1 |
Sample type |
RNA |
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|
Source name |
Zebrafish sox2/3/19a/19b knockdown embryo at 75% epiboly stage
|
Organism |
Danio rerio |
Characteristics |
tissue: whole embryo strain: TL
|
Growth protocol |
Embryos were grown in in 0.03% Red Sea salt solution.
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Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was prepared from 100 embryos using Ribo Pure kit (Ambion).
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Label |
biotin
|
Label protocol |
cRNA probes were prepared using 4 microgram total RNA with a one-cycle cDNA synthesis kit (Affymetrix).
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|
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Hybridization protocol |
10 micrograms of fragmented cRNA probes were hybridized to the GeneChip array and posthybridization staining and washing were performed according to the manufacture’s instruction (Affymetrix).
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Scan protocol |
GeneChip arrays were scanned with GS3000 scanner.
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Description |
Gene expression data from embryos injected with MOs for sox2/3/19a/19b quadruple knockdown at 75% epiboly stage.
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Data processing |
Data were processed using the RMA program.
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Submission date |
Oct 30, 2009 |
Last update date |
Oct 30, 2009 |
Contact name |
Yusuke Kamachi |
E-mail(s) |
yusuke@fbs.osaka-u.ac.jp
|
Organization name |
Osaka University
|
Department |
Graduate School of Frontier Biosciences
|
Street address |
1-3 Yamadaoka
|
City |
Suita |
State/province |
Osaka |
ZIP/Postal code |
565-0871 |
Country |
Japan |
|
|
Platform ID |
GPL1319 |
Series (1) |
GSE18830 |
B1 sox (sox2/3/19a/19b) quadruple knockdown in the zebrafish embryo |
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