|
| Status |
Public on Nov 25, 2009 |
| Title |
Estradiol minus_replicate 2_hybridization 1 |
| Sample type |
RNA |
| |
|
| Source name |
G1E-ER-GATA-1 uninduced
|
| Organism |
Mus musculus |
| Characteristics |
strain: 129/SV
cell type: G1E-ER-GATA-1
|
| Treatment protocol |
ER-GATA-1 expression was induced with 1 µM β-estradiol and RNA was collected after 24 hours.
|
| Growth protocol |
G1E-ER-GATA-1 cells (Gregory et al., 1999; Johnson et al., 2002) were maintained in Iscove’s Modified Dulbecco’s Medium (IMDM) (GIBCO) supplemented with 15% FBS (Gemini), 1% penicillin-streptomycin (GIBCO), 2 u/ml erythropoietin, 120 nM monothioglycerol (MTG) (Sigma), 0.6% conditioned medium from a Kit-ligand-producing Chinese hamster ovary (CHO) cell line and 1 µg/ml puromycin (Sigma).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using TRIzol (GIBCO/BRL) according to the manufacturer's protocol.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Illumina protocol from total RNA.
|
| |
|
| Hybridization protocol |
Hybridization to the Illumina Sentrix Expression BeadChip, staining and washing were carried out according to the manufacturer's protocol.
|
| Scan protocol |
Scanning procedures were carried out as described by Illumina.
|
| Description |
SAMPLE 3
Replicate 2, hyb 1
|
| Data processing |
Data were normalized by rank invariant normalization using the Illumina Bead Studio software suite.
|
| |
|
| Submission date |
Nov 03, 2009 |
| Last update date |
Nov 03, 2009 |
| Contact name |
Emery H. Bresnick |
| E-mail(s) |
ehbresni@facstaff.wisc.edu
|
| Phone |
608-265-6446
|
| Fax |
608-262-1257
|
| URL |
http://www.wisc.edu/molpharm/faculty/bresnick.html
|
| Organization name |
University of Wisconsin-Madison
|
| Department |
Pharmacology
|
| Lab |
Bresnick Lab
|
| Street address |
1300 University Avenue 383 MSC
|
| City |
Madison |
| State/province |
WI |
| ZIP/Postal code |
53706 |
| Country |
USA |
| |
|
| Platform ID |
GPL6103 |
| Series (2) |
| GSE18829 |
Discovering Hematopoietic Mechanisms Through Genome-Wide Analysis of GATA Factor Chromatin Occupancy |
| GSE18870 |
Analysis of global gene expression in uninduced and β-estradiol treated G1E-ER-GATA cells |
|
