To monitor gene expression profile following inhibition of novel nuclear single polypeptide RNA polymerase RNAP IV-sp (encoded in an alternative transcript from the POLRMT gene) we carried out hybridization of total RNA from mitochondrial DNA-deficient Rho0 HeLa cells with and without inhibition of POLRMT gene transcripts. The Rho0 HeLa cells were infected with recombinant lentiviral vector pLSLG expressing short hairpin RNAi complementary to Exons 3 and 17 of the POLRMT gene. 48 hours after infection total RNA was isolated and analyzed for inhibition of POLRMT transcripts by RT-PCR. Total RNA from Rho0 HeLa infected with empty pLSLG vector was used as control. The two samples of RNA were used for microarray hybridization with Affymetrix human genome U133 2.0 plus microarray. Lot batch =
