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Sample GSM468238 Query DataSets for GSM468238
Status Public on Oct 13, 2016
Title Fgf2-
Sample type RNA
 
Source name Fgf2-
Organism Mus musculus
Characteristics cell type: mouse embryonic fibroblast
gender: male
agent: none
Extracted molecule total RNA
Extraction protocol 300 ng of total DNA-free RNA was used as input for labelled cRNA synthesis (Illumina TotalPrep RNA Amplification Kit - Ambion) following the manufacturer's instructions (IVT: 12h)
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description Fgf2-
Data processing The bead intensities were mapped to gene information using BeadStudio 3.2 (Illumina), background correction was perform using the Affymetrix Robust Multi-array Analysis (RMA) background correction model, variance stabilization was performed using the log2 scaling and gene expression normalization was calculated with the quantile method implemented in the lumi package of R-Bioconductor.
 
Submission date Nov 04, 2009
Last update date Oct 13, 2016
Contact name Marcos J Ara├║zo-Bravo
E-mail(s) mararabra@yahoo.co.uk
Organization name Max Planck Institute for Molecular Biomedicine
Department Cell and Developmental Biology
Lab Computational Biology and Bioinformatics
Street address Rontgenstr. 20
City Muenster
ZIP/Postal code 48149
Country Germany
 
Platform ID GPL6885
Series (1)
GSE18888 Hit and Run Protocol with Growth Factor Enhances the Generation of Mouse Induced Pluripotent Stem Cells

Data table header descriptions
ID_REF
VALUE quantile normalized

Data table
ID_REF VALUE
ILMN_1212607 4.296479
ILMN_1212612 6.353288
ILMN_1212619 5.900504
ILMN_1212628 2.420452
ILMN_1212632 5.019582
ILMN_1212636 11.693591
ILMN_1212637 10.062369
ILMN_1212645 4.042027
ILMN_1212648 8.310960
ILMN_1212653 7.550364
ILMN_1212672 7.850183
ILMN_1212682 4.359268
ILMN_1212683 2.533547
ILMN_1212685 2.481578
ILMN_1212692 5.944951
ILMN_1212693 7.070884
ILMN_1212695 2.081128
ILMN_1212698 2.439815
ILMN_1212702 3.316408
ILMN_1212703 6.791409

Total number of rows: 25697

Table truncated, full table size 554 Kbytes.




Supplementary data files not provided
Processed data included within Sample table
Raw data are available on Series record

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