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Sample GSM468239 Query DataSets for GSM468239
Status Public on Oct 13, 2016
Title Fgf2+
Sample type RNA
 
Source name Fgf2+
Organism Mus musculus
Characteristics cell type: mouse embryonic fibroblast
gender: male
agent: Fgf2
Extracted molecule total RNA
Extraction protocol 300 ng of total DNA-free RNA was used as input for labelled cRNA synthesis (Illumina TotalPrep RNA Amplification Kit - Ambion) following the manufacturer's instructions (IVT: 12h)
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description Fgf2+
Data processing The bead intensities were mapped to gene information using BeadStudio 3.2 (Illumina), background correction was perform using the Affymetrix Robust Multi-array Analysis (RMA) background correction model, variance stabilization was performed using the log2 scaling and gene expression normalization was calculated with the quantile method implemented in the lumi package of R-Bioconductor.
 
Submission date Nov 04, 2009
Last update date Oct 13, 2016
Contact name Marcos J Ara├║zo-Bravo
E-mail(s) mararabra@yahoo.co.uk
Organization name Max Planck Institute for Molecular Biomedicine
Department Cell and Developmental Biology
Lab Computational Biology and Bioinformatics
Street address Rontgenstr. 20
City Muenster
ZIP/Postal code 48149
Country Germany
 
Platform ID GPL6885
Series (1)
GSE18888 Hit and Run Protocol with Growth Factor Enhances the Generation of Mouse Induced Pluripotent Stem Cells

Data table header descriptions
ID_REF
VALUE quantile normalized

Data table
ID_REF VALUE
ILMN_1212607 4.414758
ILMN_1212612 3.412429
ILMN_1212619 6.547062
ILMN_1212628 2.053336
ILMN_1212632 5.799574
ILMN_1212636 11.561811
ILMN_1212637 9.666823
ILMN_1212645 2.275434
ILMN_1212648 8.324636
ILMN_1212653 7.770079
ILMN_1212672 7.566093
ILMN_1212682 4.417092
ILMN_1212683 2.451960
ILMN_1212685 2.838553
ILMN_1212692 7.436559
ILMN_1212693 7.391384
ILMN_1212695 2.677867
ILMN_1212698 3.128175
ILMN_1212702 2.618789
ILMN_1212703 6.158475

Total number of rows: 25697

Table truncated, full table size 554 Kbytes.




Supplementary data files not provided
Processed data included within Sample table
Raw data are available on Series record

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