|
| Status |
Public on Jan 01, 2011 |
| Title |
900_24_CSA_1 |
| Sample type |
RNA |
| |
|
| Source name |
IMCD cultivated at 600 mosmol/kg challenged for 24h with 900 mosmol/kg + CSA rep.1
|
| Organism |
Rattus norvegicus |
| Characteristics |
tissue: Renal inner medulla
cell type: collecting duct cells
culture condition: 600 mosmol/kg
treatment group: challenged for 24h with 900 mosmol/kg + Cyclosporin A
|
| Treatment protocol |
Treated with 5 µM Cyclosporin A for 24h as indicated.
|
| Growth protocol |
Rat primary cultured inner medullary collecting duct cells cultivated at different osmolalities
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction of total RNA was performed using Qiagen RNeasy kit according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Single-stranded cDNA was generated from the amplified cRNA with the WT cDNA Synthesis Kit (Affymetrix) and then fragmented and labeled with the WT Terminal Labeling Kit (Affymetrix).
|
| |
|
| Hybridization protocol |
Samples were hybridized with GeneChip Rat Gene 1.0 ST Arrays (Affymetrix) and scanned at the University Hospital Muenster IFG Microarray Core Facility.
|
| Scan protocol |
Affymetrix Gene ChIP Scanner 3000 7G was used.
|
| Description |
Rat primary cultured inner medullary collecting duct cells
|
| Data processing |
Data were processed using XRAY software. Background correction were applied to probe intensities, gene level expression were summarized from the corrected intensities and then quantile normalized and log transformed.
|
| |
|
| Submission date |
Nov 09, 2009 |
| Last update date |
Jan 01, 2011 |
| Contact name |
Bayram Edemir |
| E-mail(s) |
bayram.edemir@uk-halle.de
|
| Phone |
+493455574980
|
| Organization name |
University Hospital Halle (Saale)
|
| Street address |
Ernst-Grube-Str. 20
|
| City |
Halle |
| ZIP/Postal code |
06120 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6247 |
| Series (1) |
| GSE18945 |
Tonicity iduced changes in gene expression in IMCD cells and the effect of Cyclosporin A |
|
