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Status |
Public on Jul 01, 2012 |
Title |
Plasmodium falciparum, co-cultured with NK cells, A |
Sample type |
RNA |
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Source name |
Plasmodium falciparum FCR3, co-cultured with NK92 cells
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Organism |
Plasmodium falciparum |
Characteristics |
strain: FCR3 csa adhesion: yes treatment: co-culture with NK92 treatment time: 24h
|
Treatment protocol |
Prior to each experiment, FCR3CSA parasites were selected for CSA adhesion by panning. Both strains were constantly synchronized with 5% sorbitol and mature trophozoite-infected erythrocytes (iRBCs) were purified by magnetic cell sorting LD columns (MACS; Miltenyi Biotec). After 24 hours co-culture of NK92 and FCR3CSA-infected erythrocytes (1:3) at 37ºC in 5% CO2, cells and parasites were separated by Ficoll gradient centrifugation.
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Growth protocol |
FCR3CSA was maintained in continuous culture as described by Trager and Jensen. NK92 Cells were kept in culture at a concentration of 0.2-0.6x106 cells/ml in alpha-MEM (75%; Sigma-Aldrich) supplemented with FBS (12,5%; Sigma-Aldrich), horse serum (12,5%; Sigma-Aldrich), L-glutamine (2 mM; Sigma-Aldrich), penicillin – streptomycin (10ml/L; Invitrogen) and recombinant human interleukin-2 (rIL-2; 10 ng/ml; Invitrogen). Saturated cultures were split 1:2 every second day.
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Extracted molecule |
total RNA |
Extraction protocol |
RNA was isolated with Trizol according to manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Standard Affymetrix labeling protocol.
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Hybridization protocol |
Standard Affymetrix Hybridization Protocol.
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Scan protocol |
Standard Affymetrix Scanning Protocol.
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Description |
Co-culture of FCR3-CSA with NK92.
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Data processing |
Signals were normalized using GC-RMA.
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Submission date |
Nov 13, 2009 |
Last update date |
Jul 01, 2012 |
Contact name |
Evelyn Boettger |
E-mail(s) |
evelyn-boettger@hotmail.de
|
Organization name |
Institute of Tropical Medicine
|
Street address |
Wilhelmstrasse 27
|
City |
Tuebingen |
ZIP/Postal code |
72074 |
Country |
Germany |
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|
Platform ID |
GPL1321 |
Series (1) |
GSE19010 |
Gene expression profiling of Plasmodium falciparum after co-culture with NK cells |
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