|
| Status |
Public on Jul 01, 2012 |
| Title |
Plasmodium falciparum, co-cultured with NK cells, C |
| Sample type |
RNA |
| |
|
| Source name |
Plasmodium falciparum FCR3, co-cultured with NK92 cells
|
| Organism |
Plasmodium falciparum |
| Characteristics |
strain: FCR3
csa adhesion: yes
treatment: co-culture with NK92
treatment time: 24h
|
| Treatment protocol |
Prior to each experiment, FCR3CSA parasites were selected for CSA adhesion by panning. Both strains were constantly synchronized with 5% sorbitol and mature trophozoite-infected erythrocytes (iRBCs) were purified by magnetic cell sorting LD columns (MACS; Miltenyi Biotec). After 24 hours co-culture of NK92 and FCR3CSA-infected erythrocytes (1:3) at 37ºC in 5% CO2, cells and parasites were separated by Ficoll gradient centrifugation.
|
| Growth protocol |
FCR3CSA was maintained in continuous culture as described by Trager and Jensen. NK92 Cells were kept in culture at a concentration of 0.2-0.6x106 cells/ml in alpha-MEM (75%; Sigma-Aldrich) supplemented with FBS (12,5%; Sigma-Aldrich), horse serum (12,5%; Sigma-Aldrich), L-glutamine (2 mM; Sigma-Aldrich), penicillin – streptomycin (10ml/L; Invitrogen) and recombinant human interleukin-2 (rIL-2; 10 ng/ml; Invitrogen). Saturated cultures were split 1:2 every second day.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated with Trizol according to manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Standard Affymetrix labeling protocol.
|
| |
|
| Hybridization protocol |
Standard Affymetrix Hybridization Protocol.
|
| Scan protocol |
Standard Affymetrix Scanning Protocol.
|
| Description |
Co-culture of FCR3-CSA with NK92.
|
| Data processing |
Signals were normalized using GC-RMA.
|
| |
|
| Submission date |
Nov 13, 2009 |
| Last update date |
Jul 01, 2012 |
| Contact name |
Evelyn Boettger |
| E-mail(s) |
evelyn-boettger@hotmail.de
|
| Organization name |
Institute of Tropical Medicine
|
| Street address |
Wilhelmstrasse 27
|
| City |
Tuebingen |
| ZIP/Postal code |
72074 |
| Country |
Germany |
| |
|
| Platform ID |
GPL1321 |
| Series (1) |
| GSE19010 |
Gene expression profiling of Plasmodium falciparum after co-culture with NK cells |
|
