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Sample GSM470405 Query DataSets for GSM470405
Status Public on Jul 01, 2012
Title Plasmodium falciparum, Ficoll control, C
Sample type RNA
 
Source name Plasmodium falciparum FCR3, Ficoll control
Organism Plasmodium falciparum
Characteristics strain: FCR3
csa adhesion: yes
developmental stage: 24h after late trophozoite stage
treatment: passed over Ficoll gradient
Treatment protocol Prior to each experiment, FCR3CSA parasites were selected for chondroitin sulfate A (CSA) adhesion by panning. Both strains were constantly synchronized with 5% sorbitol and mature trophozoite-infected erythrocytes (iRBCs) were purified by magnetic cell sorting LD columns (MACS; Miltenyi Biotec). After 24 hours culture of FCR3CSA-infected erythrocytes at 37ÂșC in 5% CO2, the parasites were separated by Ficoll gradient centrifugation.
Growth protocol FCR3CSA was maintained in continuous culture as described by Trager and Jensen.
Extracted molecule total RNA
Extraction protocol RNA was isolated with Trizol according to manufacturer's instructions.
Label biotin
Label protocol Standard Affymetrix labeling protocol.
 
Hybridization protocol Standard Affymetrix Hybridization Protocol.
Scan protocol Standard Affymetrix Scanning Protocol.
Description FCR3-CSA separated over a Ficoll gradient (Ficoll control).
Data processing Signals were normalized using GC-RMA.
 
Submission date Nov 13, 2009
Last update date Jul 01, 2012
Contact name Evelyn Boettger
E-mail(s) evelyn-boettger@hotmail.de
Organization name Institute of Tropical Medicine
Street address Wilhelmstrasse 27
City Tuebingen
ZIP/Postal code 72074
Country Germany
 
Platform ID GPL1321
Series (1)
GSE19010 Gene expression profiling of Plasmodium falciparum after co-culture with NK cells

Data table header descriptions
ID_REF
VALUE GC-RMA-normalized signal intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 8.008585
AFFX-BioB-M_at 8.454842
AFFX-BioB-3_at 8.902075
AFFX-BioC-5_at 9.895745
AFFX-BioC-3_at 9.353334
AFFX-BioDn-5_at 10.752062
AFFX-BioDn-3_at 12.733473
AFFX-CreX-5_at 13.672922
AFFX-CreX-3_at 14.35826
AFFX-DapX-5_at 5.925494
AFFX-DapX-M_at 7.263051
AFFX-DapX-3_at 9.059852
AFFX-LysX-5_at 2.6619813
AFFX-LysX-M_at 3.916462
AFFX-LysX-3_at 5.5911536
AFFX-PheX-5_at 3.6504223
AFFX-PheX-M_at 3.879164
AFFX-PheX-3_at 6.169138
AFFX-ThrX-5_at 2.8327627
AFFX-ThrX-M_at 5.152382

Total number of rows: 22769

Table truncated, full table size 643 Kbytes.




Supplementary file Size Download File type/resource
GSM470405.CEL.gz 1.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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