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Sample GSM470586 Query DataSets for GSM470586
Status Public on Jan 22, 2010
Title 3 dpi N2OSKM-infected MEFs biological replicate 1
Sample type RNA
 
Source name 3 dpi N2OSKM-infected MEFs
Organism Mus musculus
Characteristics cell type: Mouse embryonic fibroblasts (MEFs)
Treatment protocol n.a.
Growth protocol Infected MEFs were cultured at 37°C with 5% CO2 in relevant media cited in methods.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using Trizol (Invitrogen) and purified with RNeasy minikit (Qiagen).
Label Biotin
Label protocol Biotinylated cRNA were prepared from 500 ng of total RNA according to the standard protocol (TotalPrep RNA Amplification Kit, Instruction Manual, Catalog #IL1791, Illumina).
 
Hybridization protocol 1.5 ug of cRNA were hybridized to Illumina Mouse-6 Expression BeadChip v1.1 for 20 hr at 58oC on Ilumina Hybridization Oven.
Scan protocol Illumina Mouse-6 Expression BeadChip v1.1 were washed and stained with streptavidin-Cy3 before scanning using the Illumina Bead Reader.
Description Gene expression data from 3 dpi N2OSKM-infected MEFs
Data processing The data were analyzed using Bead Array suite software. Rank invariant normalization method was chosen to obtain the signal intensity.
 
Submission date Nov 13, 2009
Last update date Jan 22, 2010
Contact name Dominic Jian Chien Heng
E-mail(s) hengjcd99@gis.a-star.edu.sg
Phone 65-96734683
Organization name Genome Institute of Singapore
Department Stem Cell and Developmental Biology
Lab Stem Cell and Development Biology 6
Street address 60 Biopolis Street #02-01, Genome Building
City Singapore
ZIP/Postal code 138672
Country Singapore
 
Platform ID GPL6105
Series (3)
GSE19021 Global gene expression analyses of the Nr5a2 reprogrammed cells
GSE19022 Global gene expression analysis of OSKM / N2SKM- infected MEFs over time course
GSE19023 The Nuclear Receptor Nr5a2 can replace Oct4 in the Reprogramming of Murine Somatic Cells to Pluripotent Cells

Data table header descriptions
ID_REF Illumina probe ID
VALUE Signal intensity
DETECTION P-VALUE detection p-value

Data table
ID_REF VALUE DETECTION P-VALUE
ILMN_2417611 61.30962 0.7020726
ILMN_1238136 70.89466 0.3348446
ILMN_2721178 347.2415 0
ILMN_2458840 64.3997 0.5731865
ILMN_1230777 4234.374 0
ILMN_2537239 1061.108 0
ILMN_1246069 1866.418 0
ILMN_2524361 78.29769 0.1722798
ILMN_1243193 909.0491 0
ILMN_1232042 65.77757 0.5168394
ILMN_2543689 1734.195 0
ILMN_1233188 126.9 0
ILMN_1224596 118.2938 0
ILMN_1233643 57.10987 0.8503886
ILMN_1222139 114.9601 0
ILMN_1259789 76.48894 0.2066062
ILMN_2773981 500.8061 0
ILMN_2771349 562.8691 0
ILMN_1215157 1363.427 0
ILMN_1216623 1058.783 0

Total number of rows: 46632

Table truncated, full table size 1345 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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