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Sample GSM470907 Query DataSets for GSM470907
Status Public on Mar 31, 2010
Title Control KH2 cells treated with doxycycline
Sample type RNA
 
Source name Control KH2 cells treated with doxycycline
Organism Mus musculus
Characteristics cell type: Mouse embryonic stem cell
Treatment protocol Sox17-inducible ES cells were treated with 1μg/mL of doxycycline for 5days prior to harvesting.
Growth protocol Sox17-inducible ES cells were grown for 5days prior to harvesting. ES media consists of: 85% KO-DMEM; 15% fetal bovine serum (Hyclone); 1% L-glutamine (Invitrogen); 1% β-mercaptoethanol (Invitrogen); 1% Penicillin/Streptomycin (Invitrogen) 103IU of LIF (Chemicon). No MEFs were used in these experiments.
Extracted molecule total RNA
Extraction protocol Trizol (Invitrogen)
Label biotin
Label protocol Illumina TotalPrep RNA Amplification Kit (Ambion)
 
Hybridization protocol Illumina BeadStation (Illumina)
Scan protocol Illumina BeadStation (Illumina)
Description Control KH2 mouse embryonic stem cell
4592728087_A
Data processing Illumina BeadStudio (Illumina); rank invariant normalization, background subtraction using Illumina BeadStudio (Illumina)
 
Submission date Nov 13, 2009
Last update date Nov 17, 2009
Contact name Kathy Niakan
Organization name Harvard University
Department Stem Cell and Regenerative Biology
Lab Kevin Eggan
Street address 7 Divinity Ave
City Cambridge
State/province MA
ZIP/Postal code 02138
Country USA
 
Platform ID GPL6885
Series (1)
GSE19028 Gene Expression Analysis of Sox17-induced Mouse Embryonic Stem Cells

Data table header descriptions
ID_REF
VALUE normalized signal intensity
4592728087_A.Avg_NBEADS
4592728087_A.BEAD_STDERR
4592728087_A.Detection Pval

Data table
ID_REF VALUE 4592728087_A.Avg_NBEADS 4592728087_A.BEAD_STDERR 4592728087_A.Detection Pval
ILMN_1248788 56.52369 40 5.924755 0.03132832
ILMN_2707227 53.64878 36 5.991058 0.03383459
ILMN_2896528 5079.845 47 179.0988 0
ILMN_2721178 972.1406 36 56.63144 0
ILMN_1227723 484.6338 39 26.58317 0
ILMN_3033922 570.2182 42 25.87343 0
ILMN_3092673 2294.943 42 100.4266 0
ILMN_2730714 4290.331 47 131.4189 0
ILMN_3162224 4873.351 40 110.3783 0
ILMN_2816356 68.60964 34 10.29424 0.01253133
ILMN_2808939 1511.625 40 60.07535 0
ILMN_2634564 1496.924 35 76.65086 0
ILMN_1216623 2124.801 37 94.19689 0
ILMN_1215157 2998.272 42 121.2587 0
ILMN_1228777 5.596035 42 4.771348 0.5551379
ILMN_2737647 20.44269 26 4.018096 0.2368421
ILMN_1216425 -0.5523812 35 2.862448 0.7869674
ILMN_2734484 384.5447 40 20.01194 0
ILMN_2652961 0.3700208 44 2.725275 0.7506266
ILMN_2952292 57.3835 51 5.208668 0.03007519

Total number of rows: 25697

Table truncated, full table size 1038 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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