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Sample GSM470908 Query DataSets for GSM470908
Status Public on Mar 31, 2010
Title Control untreated KH2 cells
Sample type RNA
 
Source name Control untreated KH2 cells
Organism Mus musculus
Characteristics cell type: Mouse embryonic stem cell
Treatment protocol Sox17-inducible ES cells were treated with 1μg/mL of doxycycline for 5days prior to harvesting.
Growth protocol Sox17-inducible ES cells were grown for 5days prior to harvesting. ES media consists of: 85% KO-DMEM; 15% fetal bovine serum (Hyclone); 1% L-glutamine (Invitrogen); 1% β-mercaptoethanol (Invitrogen); 1% Penicillin/Streptomycin (Invitrogen) 103IU of LIF (Chemicon). No MEFs were used in these experiments.
Extracted molecule total RNA
Extraction protocol Trizol (Invitrogen)
Label biotin
Label protocol Illumina TotalPrep RNA Amplification Kit (Ambion)
 
Hybridization protocol Illumina BeadStation (Illumina)
Scan protocol Illumina BeadStation (Illumina)
Description Control KH2 mouse embryonic stem cell
4592728087_B
Data processing Illumina BeadStudio (Illumina); rank invariant normalization, background subtraction using Illumina BeadStudio (Illumina)
 
Submission date Nov 13, 2009
Last update date Nov 17, 2009
Contact name Kathy Niakan
Organization name Harvard University
Department Stem Cell and Regenerative Biology
Lab Kevin Eggan
Street address 7 Divinity Ave
City Cambridge
State/province MA
ZIP/Postal code 02138
Country USA
 
Platform ID GPL6885
Series (1)
GSE19028 Gene Expression Analysis of Sox17-induced Mouse Embryonic Stem Cells

Data table header descriptions
ID_REF
VALUE normalized signal intensity
4592728087_B.Avg_NBEADS
4592728087_B.BEAD_STDERR
4592728087_B.Detection Pval

Data table
ID_REF VALUE 4592728087_B.Avg_NBEADS 4592728087_B.BEAD_STDERR 4592728087_B.Detection Pval
ILMN_1248788 37.95445 55 6.058743 0.04511278
ILMN_2707227 43.9495 41 5.317504 0.03007519
ILMN_2896528 3366.217 48 126.7813 0
ILMN_2721178 651.8413 37 27.00132 0
ILMN_1227723 339.8044 52 16.33845 0
ILMN_3033922 387.6963 45 17.04757 0
ILMN_3092673 1749.776 48 80.39149 0
ILMN_2730714 3952.859 41 157.4575 0
ILMN_3162224 5180.304 50 213.3247 0
ILMN_2816356 72.36887 46 7.084417 0.006265664
ILMN_2808939 1277.133 36 46.91968 0
ILMN_2634564 1409.348 29 82.3013 0
ILMN_1216623 1973.804 32 94.90009 0
ILMN_1215157 2708.469 43 98.52795 0
ILMN_1228777 -2.621174 40 4.326831 0.5927318
ILMN_2737647 3.845906 37 5.135846 0.3721805
ILMN_1216425 -7.52813 39 3.663 0.7531328
ILMN_2734484 384.6893 37 22.0624 0
ILMN_2652961 -5.531102 46 3.507327 0.6867168
ILMN_2952292 77.22528 34 6.193053 0.003759399

Total number of rows: 25697

Table truncated, full table size 1038 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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