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Sample GSM470910 Query DataSets for GSM470910
Status Public on Mar 31, 2010
Title Untreated Sox17-inducible ES cells
Sample type RNA
 
Source name Untreated Sox17-inducible ES cells
Organism Mus musculus
Characteristics cell type: Mouse embryonic stem cell
Treatment protocol Sox17-inducible ES cells were treated with 1μg/mL of doxycycline for 5days prior to harvesting.
Growth protocol Sox17-inducible ES cells were grown for 5days prior to harvesting. ES media consists of: 85% KO-DMEM; 15% fetal bovine serum (Hyclone); 1% L-glutamine (Invitrogen); 1% β-mercaptoethanol (Invitrogen); 1% Penicillin/Streptomycin (Invitrogen) 103IU of LIF (Chemicon). No MEFs were used in these experiments.
Extracted molecule total RNA
Extraction protocol Trizol (Invitrogen)
Label biotin
Label protocol Illumina TotalPrep RNA Amplification Kit (Ambion)
 
Hybridization protocol Illumina BeadStation (Illumina)
Scan protocol Illumina BeadStation (Illumina)
Description Sox17-inducible mouse embryonic stem cell
4592728087_D
Data processing Illumina BeadStudio (Illumina); rank invariant normalization, background subtraction using Illumina BeadStudio (Illumina)
 
Submission date Nov 13, 2009
Last update date Nov 17, 2009
Contact name Kathy Niakan
Organization name Harvard University
Department Stem Cell and Regenerative Biology
Lab Kevin Eggan
Street address 7 Divinity Ave
City Cambridge
State/province MA
ZIP/Postal code 02138
Country USA
 
Platform ID GPL6885
Series (1)
GSE19028 Gene Expression Analysis of Sox17-induced Mouse Embryonic Stem Cells

Data table header descriptions
ID_REF
VALUE normalized signal intensity
4592728087_D.Avg_NBEADS
4592728087_D.BEAD_STDERR
4592728087_D.Detection Pval

Data table
ID_REF VALUE 4592728087_D.Avg_NBEADS 4592728087_D.BEAD_STDERR 4592728087_D.Detection Pval
ILMN_1248788 29.24963 36 6.663057 0.1077694
ILMN_2707227 47.61282 45 6.209577 0.02631579
ILMN_2896528 3943.176 54 106.1145 0
ILMN_2721178 843.433 43 40.76013 0
ILMN_1227723 259.8298 42 17.42315 0
ILMN_3033922 590.1068 38 30.28068 0
ILMN_3092673 2067.782 38 101.0265 0
ILMN_2730714 3358.771 42 151.1734 0
ILMN_3162224 4919.491 38 236.5855 0
ILMN_2816356 98.87595 38 10.8114 0
ILMN_2808939 1407.217 35 55.23564 0
ILMN_2634564 1032.711 41 34.56503 0
ILMN_1216623 1677.886 45 66.3821 0
ILMN_1215157 2071.217 47 73.49059 0
ILMN_1228777 -3.448014 36 4.398688 0.7343358
ILMN_2737647 9.88747 23 6.389233 0.3220551
ILMN_1216425 -5.723444 46 2.934117 0.8182957
ILMN_2734484 501.7894 36 27.46936 0
ILMN_2652961 -10.68862 44 2.848387 0.9473684
ILMN_2952292 60.88914 55 4.746868 0.01253133

Total number of rows: 25697

Table truncated, full table size 1038 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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