NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM470911 Query DataSets for GSM470911
Status Public on Mar 31, 2010
Title Sox17-inducible-line 2 ES cells treated with doxycyline
Sample type RNA
 
Source name Sox17-inducible-line 2 ES cells treated with doxycyline
Organism Mus musculus
Characteristics cell type: Mouse embryonic stem cell
Treatment protocol Sox17-inducible ES cells were treated with 1μg/mL of doxycycline for 5days prior to harvesting.
Growth protocol Sox17-inducible ES cells were grown for 5days prior to harvesting. ES media consists of: 85% KO-DMEM; 15% fetal bovine serum (Hyclone); 1% L-glutamine (Invitrogen); 1% β-mercaptoethanol (Invitrogen); 1% Penicillin/Streptomycin (Invitrogen) 103IU of LIF (Chemicon). No MEFs were used in these experiments.
Extracted molecule total RNA
Extraction protocol Trizol (Invitrogen)
Label biotin
Label protocol Illumina TotalPrep RNA Amplification Kit (Ambion)
 
Hybridization protocol Illumina BeadStation (Illumina)
Scan protocol Illumina BeadStation (Illumina)
Description Sox17-inducible mouse embryonic stem cell_line 2
4592728087_E
Data processing Illumina BeadStudio (Illumina); rank invariant normalization, background subtraction using Illumina BeadStudio (Illumina)
 
Submission date Nov 13, 2009
Last update date Nov 17, 2009
Contact name Kathy Niakan
Organization name Harvard University
Department Stem Cell and Regenerative Biology
Lab Kevin Eggan
Street address 7 Divinity Ave
City Cambridge
State/province MA
ZIP/Postal code 02138
Country USA
 
Platform ID GPL6885
Series (1)
GSE19028 Gene Expression Analysis of Sox17-induced Mouse Embryonic Stem Cells

Data table header descriptions
ID_REF
VALUE normalized signal intensity
4592728087_E.Avg_NBEADS
4592728087_E.BEAD_STDERR
4592728087_E.Detection Pval

Data table
ID_REF VALUE 4592728087_E.Avg_NBEADS 4592728087_E.BEAD_STDERR 4592728087_E.Detection Pval
ILMN_1248788 14.65929 55 3.99029 0.1892231
ILMN_2707227 59.15562 39 7.636231 0.0112782
ILMN_2896528 4873.832 45 138.2814 0
ILMN_2721178 1066.291 38 54.71965 0
ILMN_1227723 491.7791 55 21.35249 0
ILMN_3033922 961.1362 65 35.61867 0
ILMN_3092673 3342.031 46 117.4241 0
ILMN_2730714 4537.497 55 176.9767 0
ILMN_3162224 4602.088 47 166.7414 0
ILMN_2816356 74.34395 33 11.06424 0.006265664
ILMN_2808939 1692.556 27 84.53242 0
ILMN_2634564 1218.371 41 93.10149 0
ILMN_1216623 1686.107 48 69.85242 0
ILMN_1215157 2270.17 37 99.20627 0
ILMN_1228777 -12.14824 33 4.545666 0.8132832
ILMN_2737647 13.50198 33 6.353296 0.2055138
ILMN_1216425 -9.034336 51 3.675605 0.7180451
ILMN_2734484 513.0826 43 29.15495 0
ILMN_2652961 -9.886209 42 4.219292 0.7418546
ILMN_2952292 57.05103 50 6.664714 0.0112782

Total number of rows: 25697

Table truncated, full table size 1041 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap