NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM470912 Query DataSets for GSM470912
Status Public on Mar 31, 2010
Title Untreated Sox17-inducible-line 2 ES cells
Sample type RNA
 
Source name Untreated Sox17-inducible-line 2 ES cells
Organism Mus musculus
Characteristics cell type: Mouse embryonic stem cell
Treatment protocol Sox17-inducible ES cells were treated with 1μg/mL of doxycycline for 5days prior to harvesting.
Growth protocol Sox17-inducible ES cells were grown for 5days prior to harvesting. ES media consists of: 85% KO-DMEM; 15% fetal bovine serum (Hyclone); 1% L-glutamine (Invitrogen); 1% β-mercaptoethanol (Invitrogen); 1% Penicillin/Streptomycin (Invitrogen) 103IU of LIF (Chemicon). No MEFs were used in these experiments.
Extracted molecule total RNA
Extraction protocol Trizol (Invitrogen)
Label biotin
Label protocol Illumina TotalPrep RNA Amplification Kit (Ambion)
 
Hybridization protocol Illumina BeadStation (Illumina)
Scan protocol Illumina BeadStation (Illumina)
Description Sox17-inducible mouse embryonic stem cell_line 2
4592728087_F
Data processing Illumina BeadStudio (Illumina); rank invariant normalization, background subtraction using Illumina BeadStudio (Illumina)
 
Submission date Nov 13, 2009
Last update date Nov 17, 2009
Contact name Kathy Niakan
Organization name Harvard University
Department Stem Cell and Regenerative Biology
Lab Kevin Eggan
Street address 7 Divinity Ave
City Cambridge
State/province MA
ZIP/Postal code 02138
Country USA
 
Platform ID GPL6885
Series (1)
GSE19028 Gene Expression Analysis of Sox17-induced Mouse Embryonic Stem Cells

Data table header descriptions
ID_REF
VALUE normalized signal intensity
4592728087_F.Avg_NBEADS
4592728087_F.BEAD_STDERR
4592728087_F.Detection Pval

Data table
ID_REF VALUE 4592728087_F.Avg_NBEADS 4592728087_F.BEAD_STDERR 4592728087_F.Detection Pval
ILMN_1248788 56.79938 39 8.16163 0.01503759
ILMN_2707227 41.92706 37 6.222567 0.04010025
ILMN_2896528 4318.186 41 169.7725 0
ILMN_2721178 894.2798 47 41.63644 0
ILMN_1227723 397.9955 41 22.9763 0
ILMN_3033922 805.3557 44 39.07542 0
ILMN_3092673 3058.155 42 121.9252 0
ILMN_2730714 4116.181 50 149.3421 0
ILMN_3162224 5279.171 36 243.9527 0
ILMN_2816356 68.67682 37 7.657039 0.006265664
ILMN_2808939 1721.016 43 71.66529 0
ILMN_2634564 1261.277 35 52.23335 0
ILMN_1216623 1786.482 39 71.19991 0
ILMN_1215157 2536.237 49 96.34795 0
ILMN_1228777 -10.03952 41 3.214649 0.8922306
ILMN_2737647 7.501056 44 5.176888 0.3634085
ILMN_1216425 -1.225277 41 4.835047 0.5902256
ILMN_2734484 586.4502 38 33.45401 0
ILMN_2652961 -6.701105 40 4.24263 0.7944862
ILMN_2952292 69.73546 44 5.473991 0.006265664

Total number of rows: 25697

Table truncated, full table size 1040 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap