|
| Status |
Public on Jun 01, 2010 |
| Title |
ES_EB-28d_REP2 |
| Sample type |
RNA |
| |
|
| Source name |
ES cells, EB culture for 28 days
|
| Organism |
Mus musculus |
| Characteristics |
cell type: embryonic stem (ES) cells
differentiation time: 28 days
|
| Treatment protocol |
Culture in embryoid bodies with hepatic differentiation cocktail (details in the manuscript).
|
| Growth protocol |
Standard growth protocol for each cell type.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extraction was performed using the miRNeasy kit (Qiagen). RNA qualitative and quantitative assessment was performed using the Bioanalyzer 2100 (Agilent Tecnologies).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays starting from 500ng of total RNA.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol.
|
| Scan protocol |
Standard Illumina scanning protocol.
|
| Description |
ES cells, EB culture for 28 days.
|
| Data processing |
The data were normalized using cubic Spline normalization with the Illumina GenomeStudio software.
Non-normalized data is available in the 'GSE19044_non-normalized.txt' file, which is linked to the GSE19044 Series record as a supplementary file.
|
| |
|
| Submission date |
Nov 16, 2009 |
| Last update date |
Nov 16, 2009 |
| Contact name |
Enzo Medico |
| E-mail(s) |
enzo.medico@ircc.it
|
| Phone |
+39-011-9933234
|
| Organization name |
Candiolo Cancer Institute, University of Torino
|
| Department |
Oncology
|
| Lab |
Laboratory of Oncogenomics
|
| Street address |
Strada Prov. 142, km 3,95
|
| City |
Candiolo |
| State/province |
TO |
| ZIP/Postal code |
10060 |
| Country |
Italy |
| |
|
| Platform ID |
GPL6887 |
| Series (1) |
| GSE19044 |
Hepatocytes obtained from mouse GPSCs are functional and show similar expression profiles to fetal primary hepatocytes |
|
