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Sample GSM471337 Query DataSets for GSM471337
Status Public on Jun 01, 2010
Title GPSC-A_EB-14d_REP2
Sample type RNA
 
Source name GPSC-A cells, EB culture for 14 days
Organism Mus musculus
Characteristics cell type: germline cell-derived pluripotent stem cells (GPSCs), Lot A
differentiation time: 14 days
Treatment protocol Culture in embryoid bodies with hepatic differentiation cocktail (details in the manuscript).
Growth protocol Standard growth protocol for each cell type.
Extracted molecule total RNA
Extraction protocol Total RNA extraction was performed using the miRNeasy kit (Qiagen). RNA qualitative and quantitative assessment was performed using the Bioanalyzer 2100 (Agilent Tecnologies).
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays starting from 500ng of total RNA.
 
Hybridization protocol Standard Illumina hybridization protocol.
Scan protocol Standard Illumina scanning protocol.
Description GPSC-A cells, EB culture for 14 days.
Data processing The data were normalized using cubic Spline normalization with the Illumina GenomeStudio software.

Non-normalized data is available in the 'GSE19044_non-normalized.txt' file, which is linked to the GSE19044 Series record as a supplementary file.
 
Submission date Nov 16, 2009
Last update date Nov 16, 2009
Contact name Enzo Medico
E-mail(s) enzo.medico@ircc.it
Phone +39-011-9933234
Organization name Candiolo Cancer Institute, University of Torino
Department Oncology
Lab Laboratory of Oncogenomics
Street address Strada Prov. 142, km 3,95
City Candiolo
State/province TO
ZIP/Postal code 10060
Country Italy
 
Platform ID GPL6887
Series (1)
GSE19044 Hepatocytes obtained from mouse GPSCs are functional and show similar expression profiles to fetal primary hepatocytes

Data table header descriptions
ID_REF
VALUE Cubic spline normalized expression data
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_2735294 149.4624 0.0448718
ILMN_2417611 122.7755 0.6121795
ILMN_2545897 154.4216 0.02884615
ILMN_2762289 122.3708 0.6239316
ILMN_1248788 177.5672 0.00534188
ILMN_2707227 125.0495 0.4978632
ILMN_2896528 6614.137 0
ILMN_2721178 3867.691 0
ILMN_1227723 620.6617 0
ILMN_2458837 117.0229 0.8301282
ILMN_3033922 1164.854 0
ILMN_3092673 3807.078 0
ILMN_1230777 1785.845 0
ILMN_2730714 3443.862 0
ILMN_1246069 7479.892 0
ILMN_1232042 127.4932 0.4059829
ILMN_1243193 550.8701 0
ILMN_2524361 135.8767 0.1655983
ILMN_1233188 145.7585 0.06944445
ILMN_2543688 124.3747 0.5299146

Total number of rows: 45281

Table truncated, full table size 1325 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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