|
| Status |
Public on Feb 15, 2010 |
| Title |
Liver_KO_2 |
| Sample type |
RNA |
| |
|
| Source name |
Liver from knockout mice collected 24 hours after fasting
|
| Organism |
Mus musculus |
| Characteristics |
genotype: Cav1 KO
tissue: Liver
|
| Treatment protocol |
Mice were kept with access to food and water ad libitum with cycles of light and dark of 12 hours. Sacrifice of the animals and harvest of the tissues happened 24 hours after food withdrawal for the liver and 11 hours after food withdrawal for the adipose tissue. Tissues were cleaned from blood with cold PBS and rapidly frozen in liquid nitrogen. Then, we extracted RNA following RNAeasy RNA kit purification instructions. Once purified, total RNA was store at -80 until its analysis by microarray.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Quiagen RNAeasy purification kit.
|
| Label |
Biotin
|
| Label protocol |
For labeling we followed Illumina TotalPrep RNA Amplification Kit
|
| |
|
| Hybridization protocol |
Illumina manufacturer's instructions
|
| Scan protocol |
Illumina manufacturer's instructions
|
| Description |
n/a
|
| Data processing |
data was extracted with Bead Studio, then it was imported in R and read with the Lumi package, RMA-like background corrected with the affy package, quantile normalized with the Lumi package and then analysed with LIMMA. All these packages are part of Bioconductor.
|
| |
|
| Submission date |
Nov 16, 2009 |
| Last update date |
Nov 16, 2009 |
| Contact name |
Milena Gongora |
| E-mail(s) |
milenagongora@gmail.com
|
| Organization name |
Institute for Molecular Biosciences
|
| Street address |
bld 80, services rd
|
| City |
St. Lucia |
| State/province |
QLD |
| ZIP/Postal code |
4072 |
| Country |
Australia |
| |
|
| Platform ID |
GPL6333 |
| Series (1) |
| GSE19045 |
Effects of Caveolin 1 knockout in mice |
|
