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Sample GSM471597 Query DataSets for GSM471597
Status Public on Feb 28, 2014
Title wk12_DEN100__DEN_112
Sample type RNA
 
Source name rat liver, cirrhosis (DEN 100mg, week 12)
Organism Rattus norvegicus
Characteristics strain: Wistar
sex: male
tissue: liver
disease status: cirrhosis
Treatment protocol Animals received humane care according to the criteria outlined in the “Guide for the Care and Use of Laboratory Animals” of the National Academy of Sciences. All animals were maintained in accordance with the institutional guidelines of the Massachusetts General Hospital Subcommittee on Research Animal Care. Male Wistar rats received weekly IP injections of diethylnitrosamine (DEN) at 50 mg/kg, 100mg/kg, or vehicle control (PBS) for 18 weeks. A subset of rats received either daily (5X a week) IP injections of 2 mg/kg erlotinib or vehicle control during weeks 13 - 18. In a separate study, the erlotinib dose was lowered to 0.5 mg/kg. The vehicle groups from the two studies were not significantly different so they were combined together for analysis. Rats were weighed at the end of each week. Animals were sacrificed at 9, 13 and 19 weeks after a one-week washout period to eliminate acute effects of DEN. At the time of sacrifice, the non-tumor liver tissues were collected in RNase-free tubes and snap-frozen in liquid nitrogen. Frozen tissues were stored at -80°C until RNA extraction.
Extracted molecule total RNA
Extraction protocol Using frozen non-tumor liver tissues, total RNA was extracted using TRIzol (Invitrogen).
Label Cy5
Label protocol Refer to the Illumina beadarray Assay System Manual
 
Hybridization protocol Refer to the Illumina beadarray Assay System Manual
Scan protocol Refer to the Illumina beadarray Assay System Manual
Description wk12_DEN100__DEN_112
Data processing For each probe, replicate mesurements were averaged after removing outliers. Data were were then normalized using the cubic spline algorithm using GenePattern IlluminaNormalizer module.
 
Submission date Nov 17, 2009
Last update date Feb 28, 2014
Contact name Yujin Hoshida
E-mail(s) Yujin.Hoshida@UTSouthwestern.edu
Organization name University of Texas Southwestern Medical Center
Street address 5323 Harry Hines Blvd
City Dallas
State/province TX
ZIP/Postal code 75390
Country USA
 
Platform ID GPL6101
Series (2)
GSE19057 Gene expression profile of liver tissue in low-dose, repeated diethylnitrosamine (DEN)-treated rat treated with erlotinib
GSE27641 EGFR inhibition prevents liver cirrhosis and hepatocellular carcinoma

Data table header descriptions
ID_REF
VALUE cubic spline normalized signal intensity

Data table
ID_REF VALUE
ILMN_1369537 153.7160082
ILMN_1358051 136.6860309
ILMN_1361746 317.5286389
ILMN_1351370 150.389398
ILMN_1357910 1218.160386
ILMN_1352975 477.1771411
ILMN_1368384 235.5480751
ILMN_1355285 133.3872333
ILMN_1376898 203.0575323
ILMN_1368325 181.0813034
ILMN_1353563 163.6494192
ILMN_1348794 1041.82552
ILMN_1364273 164.9235137
ILMN_1369433 1120.868768
ILMN_1349007 273.5545588
ILMN_1374156 136.0301426
ILMN_1360232 182.884513
ILMN_1355155 1085.092891
ILMN_1362563 126.1759787
ILMN_1355815 137.8225315

Total number of rows: 22523

Table truncated, full table size 546 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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