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Sample GSM471610 Query DataSets for GSM471610
Status Public on Feb 28, 2014
Title wk18_DEN50_Erlotinib2_DEN_31
Sample type RNA
Source name rat liver, cirrhosis (DEN 50mg), treated with erlotinib 2mg (week 18)
Organism Rattus norvegicus
Characteristics strain: Wistar
sex: male
tissue: liver
disease status: cirrhosis
treatment group: treated with erlotinib 2mg
Treatment protocol Animals received humane care according to the criteria outlined in the “Guide for the Care and Use of Laboratory Animals” of the National Academy of Sciences. All animals were maintained in accordance with the institutional guidelines of the Massachusetts General Hospital Subcommittee on Research Animal Care. Male Wistar rats received weekly IP injections of diethylnitrosamine (DEN) at 50 mg/kg, 100mg/kg, or vehicle control (PBS) for 18 weeks. A subset of rats received either daily (5X a week) IP injections of 2 mg/kg erlotinib or vehicle control during weeks 13 - 18. In a separate study, the erlotinib dose was lowered to 0.5 mg/kg. The vehicle groups from the two studies were not significantly different so they were combined together for analysis. Rats were weighed at the end of each week. Animals were sacrificed at 9, 13 and 19 weeks after a one-week washout period to eliminate acute effects of DEN. At the time of sacrifice, the non-tumor liver tissues were collected in RNase-free tubes and snap-frozen in liquid nitrogen. Frozen tissues were stored at -80°C until RNA extraction.
Extracted molecule total RNA
Extraction protocol Using frozen non-tumor liver tissues, total RNA was extracted using TRIzol (Invitrogen).
Label Cy5
Label protocol Refer to the Illumina beadarray Assay System Manual
Hybridization protocol Refer to the Illumina beadarray Assay System Manual
Scan protocol Refer to the Illumina beadarray Assay System Manual
Description wk18_DEN50_Erlotinib2_DEN_31
Data processing For each probe, replicate mesurements were averaged after removing outliers. Data were were then normalized using the cubic spline algorithm using GenePattern IlluminaNormalizer module.
Submission date Nov 17, 2009
Last update date Feb 28, 2014
Contact name Yujin Hoshida
Organization name University of Texas Southwestern Medical Center
Street address 5323 Harry Hines Blvd
City Dallas
State/province TX
ZIP/Postal code 75390
Country USA
Platform ID GPL6101
Series (2)
GSE19057 Gene expression profile of liver tissue in low-dose, repeated diethylnitrosamine (DEN)-treated rat treated with erlotinib
GSE27641 EGFR inhibition prevents liver cirrhosis and hepatocellular carcinoma

Data table header descriptions
VALUE cubic spline normalized signal intensity

Data table
ILMN_1369537 158.2827697
ILMN_1358051 121.8837395
ILMN_1361746 387.8473049
ILMN_1351370 142.1174934
ILMN_1357910 775.412519
ILMN_1352975 526.2624999
ILMN_1368384 134.3039208
ILMN_1355285 130.9205663
ILMN_1376898 256.2900542
ILMN_1368325 172.8085638
ILMN_1353563 191.3939675
ILMN_1348794 2186.242654
ILMN_1364273 159.1014123
ILMN_1369433 1718.331416
ILMN_1349007 404.6313823
ILMN_1374156 129.0390585
ILMN_1360232 144.447504
ILMN_1355155 2171.27776
ILMN_1362563 141.166892
ILMN_1355815 146.02281

Total number of rows: 22523

Table truncated, full table size 548 Kbytes.

Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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