|
| Status |
Public on Jan 25, 2011 |
| Title |
kidney_double-transgenic_rat_female_rep3 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
kidney, double-transgenic_rat, female
|
| Organism |
Rattus norvegicus |
| Characteristics |
tissue: kidney
strain: double-transgenic_rat
gender: female
age: 7 weeks
|
| Growth protocol |
Three-week old dTGR and SD were purchased from RCC (Füllinsdorf, Switzerland) and were sacrified at seven weeks.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA extracted using RNeasy Mini Kit (Qiagen, Hilden, Germany) following the manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
1 µg of totRNA were labeled using the low RNA Input Linear Amplification Kit PLUS (Agilent, Austin TX, USA) following the manufacturer's instructions.
|
| |
|
| Channel 2 |
| Source name |
Stratagene Reference
|
| Organism |
Rattus norvegicus |
| Characteristics |
reference: Pool of 14 rat cell types
|
| Growth protocol |
Three-week old dTGR and SD were purchased from RCC (Füllinsdorf, Switzerland) and were sacrified at seven weeks.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA extracted using RNeasy Mini Kit (Qiagen, Hilden, Germany) following the manufacturer's instructions
|
| Label |
Cy5
|
| Label protocol |
1 µg of totRNA were labeled using the low RNA Input Linear Amplification Kit PLUS (Agilent, Austin TX, USA) following the manufacturer's instructions.
|
| |
|
| |
| Hybridization protocol |
a pool of 825 ng of Cy3 labeled aRNA (examined sample) and 825 ng of Cy5 labeled aRNA rat reference (Stratagene, Agilent, Austin TX, USA) were hybridized using the Agilent Hybridization kit (Agilent, Austin TX, USA) according to the manufacturer's instructions. Hybridization was performed for 17 hours and washed according to Agilent's instructions.
|
| Scan protocol |
Scanned on an Agilent G2505B scanner using Agilent scan control (version 7.0)
Images were quantified using Agilent Feature Extraction Software (version 9.5.3.1).
|
| Description |
no additional information
|
| Data processing |
BioConductor package vsn was used for background subtraction, variance-stabilizing normalization and glog transformation
|
| |
|
| Submission date |
Nov 17, 2009 |
| Last update date |
Apr 19, 2011 |
| Contact name |
Axel Klenk |
| E-mail(s) |
axel.klenk@idorsia.com
|
| Organization name |
Idorsia Pharmaceuticals Ltd.
|
| Department |
Research Informatics
|
| Street address |
Hegenheimermattweg 91
|
| City |
Allschwil |
| ZIP/Postal code |
4123 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL4135 |
| Series (1) |
| GSE19058 |
Identification of cathepsin L as a potential sex-specific biomarker for renal damage |
|
