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Sample GSM47344 Query DataSets for GSM47344
Status Public on Apr 04, 2005
Title SkeletalMuscle:Thigh, Female (MoSM.FeTh)
Sample type MPSS
 
Source name SkeletalMuscle:Thigh, Female total RNA
Organism Mus musculus
Extracted molecule total RNA
 
Description Strain: C57BL6
SkeletalMuscle:Thigh, Female total RNA (MoSM.FeTh) received from National Human Genome Research Institute was analyzed through Bioanalyzer and passed our quality control test. The mRNA was processed according to the MPSS protocol as outlined in the previous publications (Brenner, S., et al. (2000) Proc Natl Acad Sci U S A 97(4): 1665-1670 and Brenner, S. et al., Nat. Biotechnol 18(6): 630-634). Briefly, the mRNA was reverse transcribed and the cDNA was digested with Dpn II. The 20 bases adjacent to the 3? most Dpn II site was cloned into a Megaclone vector. The resulting library was amplified and loaded onto microbeads. About 1.6 million microbeads were loaded into each flow cell and the signature sequences were determined by a series of enzymatic reactions as outlined in the above publications. The abundance for each signature was converted to transcripts per million (tpm) for the purpose of comparisons between samples.
Cells/tissue:
Library MoSM.FeTh.sig21
Cell type SkeletalMuscle:Thigh, Female
Source NHGRI
RNA isolation LYNX mRNA QC passed
cDNA library:
Library DpnII restriction - (signature cloning using MmeI)
Sequence length 20 bp
MPSS:
runs
MoSM.FeTh_sig21.5418F.a-20 2/28/2005 465810 4777 QC Passed
MoSM.FeTh_sig21.5418F.d-20 3/22/2005 449998 5537 QC Passed
MoSM.FeTh_sig21.5418F.e-20 3/23/2005 346950 3295 QC Passed
MoSM.FeTh_sig21.5418W.a-20 2/28/2005 510428 6544 QC Passed
MoSM.FeTh_sig21.5418W.c-20 3/14/2005 492804 6236 QC Passed
Run group:
Total Beads successfully sequenced - 2265990
Processed Signatures - 8867
 
Submission date Apr 04, 2005
Last update date Dec 14, 2005
Contact name Daixing Zhou
E-mail(s) dzhou@solexa.com
Phone 510-670-9441
Fax 510-670-9302
URL http://www.solexa.com
Organization name Solexa
Department Bioinformatics
Street address 25861 Industrial Blvd
City Hayward
State/province CA
ZIP/Postal code 94545
Country USA
 
Platform ID GPL1010
Series (1)
GSE1581 MPSS mouse transcriptome analysis project

Data table header descriptions
ID_REF Signature - 20 nt sequence including DpnII site (GATC)
VALUE Mean abundance for a signature derived from all MPSS runs for the sample, in transcripts per million (TPM)
ST_DEV Standard deviation of the mean abundance from multiple MPSS runs
REP Replication_global


Total number of rows: 5938

Table truncated, full table size 175 Kbytes.




Supplementary file Size Download File type/resource
GSM47344.raw.txt.gz 117.8 Kb (ftp)(http) TXT

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