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Sample GSM473742 Query DataSets for GSM473742
Status Public on Feb 12, 2010
Title 4427941023_E
Sample type RNA
 
Source name C17.2 murine neural stem cells transduced with a constitutively active mutant of STAT3 and CEBPB both cloned in retroviral vectors
Organism Mus musculus
Characteristics cell type/line: C17.2
transduction: STAT3C and CEBPB retroviral vectors
Extracted molecule total RNA
Extraction protocol TRIZOL followed by MagMAX96 for microarrays total RNA isolation kit (Ambion)
Label biotin
Label protocol 200 ng total RNA were processed according to Illumina TotalPrep RNA amplification kit (Ambion)
 
Hybridization protocol According to Illumina whole-genome gene expression direct hybridization assay guide
Scan protocol The arrays were scanned using an iScan (Illumina). Images were segmented and quantified by BeadStudio (Illumina).
Description STAT3C and CEBPB retrovirus transduction of C17.2 murine neural stem cells. 200 ng of total RNA were hybridized in Illumina MouseWG-6v2 expression arrays.
Data processing BeadStudio-based background subtraction; followed by variance stabilization transformation and robust spline normalization as implemented in lumi R package (Bioconductor)
 
Submission date Nov 20, 2009
Last update date Feb 11, 2010
Contact name Mariano Javier Alvarez
Organization name Columbia University
Department Systems Biology
Lab Systems Biology
Street address 1130 St. Nicholas ave.
City New York
State/province NY
ZIP/Postal code 10032
Country USA
 
Platform ID GPL6887
Series (2)
GSE19113 A transcriptional module initiates and maintains mesenchymal transformation in brain tumors [mouse data]
GSE20281 The transcriptional network for mesenchymal transformation of brain tumours

Data table header descriptions
ID_REF Probe identifiers, each of which is unique to a specific probe.
VALUE Signal intensity level for each probe.
DETECTION Detection p-value as estimated by BeadStudio.

Data table
ID_REF VALUE DETECTION
ILMN_2417611 6.92980721355581 0.4252137
ILMN_2762289 6.88195714281539 0.8760684
ILMN_2896528 9.88079632823736 0
ILMN_2721178 8.48594389930815 0
ILMN_2458837 6.90490400623609 0.6495727
ILMN_3033922 8.71029612850476 0
ILMN_3092673 9.62375463935368 0
ILMN_1230777 9.13006663817455 0
ILMN_1246069 9.36931241133193 0
ILMN_1232042 6.91028327997587 0.6025641
ILMN_1243193 9.65369850589595 0
ILMN_2524361 6.90050349427541 0.707265
ILMN_1242440 6.8983808327019 0.7275641
ILMN_1233188 6.87712756645109 0.9049146
ILMN_2543688 6.89167976388936 0.7873932
ILMN_1259789 7.35512967946499 0.001068376
ILMN_2816356 7.14533259209331 0.0224359
ILMN_1224596 7.16077106003696 0.01709402
ILMN_1233643 7.10241903902797 0.03952992
ILMN_2808939 8.30860909510605 0

Total number of rows: 45281

Table truncated, full table size 1683 Kbytes.




Supplementary file Size Download File type/resource
GSM473742.txt.gz 741.2 Kb (ftp)(http) TXT
Processed data included within Sample table

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