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Sample GSM4745860 Query DataSets for GSM4745860
Status Public on Aug 26, 2020
Title LP-1 WT-DMSO_rep1
Sample type SRA
 
Source name LP-1WT_DMSO1
Organism Homo sapiens
Characteristics cell type: B lymphoblast
tumor type: mutiple myeloma
cell line: LP-1
phenotype: WT
treatment: untreated
Treatment protocol Cell was 25nM SI-2 treated for 24 hours.
Growth protocol LP-1 wild-type cell and bortezomib-resistant cell were cultured in RPMI-1640 medium with 10% FBS.
Extracted molecule total RNA
Extraction protocol RNA was harvested using Trizol reagent.
RNA libraries were prepared for sequencing using BGI protocols
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model BGISEQ-500
 
Data processing Basecalls performed using BGISEQ-500
SOAPnuke was used to get the "clean reads".
Mapping clean reads to hg19 whole genome using BWA.
Bowtie2 was used to get the mapping ratio.
Fragments Per Kilobase Million (FPKM) was calculated based on the RSEM.
Genome_build: hg19
Supplementary_files_format_and_content: tab-delimited text files include fpkm values for each Sample
 
Submission date Aug 25, 2020
Last update date Aug 26, 2020
Contact name Liu Zhiqiang
E-mail(s) zhiqiangliu@tmu.edu.cn
Organization name Tianjin Medical University
Street address 22 Qxiangtai Rd
City Tianjin
ZIP/Postal code 300070
Country China
 
Platform ID GPL23227
Series (2)
GSE156871 Targeting steroid receptor co-activator 3 sensitizes myeloma cell to proteasome inhibitor treatment through NSD2-mediated phase separation and chromatin remodeling [RNA-Seq]
GSE156872 Targeting steroid receptor co-activator 3 sensitizes myeloma cell to proteasome inhibitor treatment through NSD2-mediated phase separation and chromatin remodeling
Relations
SRA SRX9006286
BioSample SAMN15904266

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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