|
Status |
Public on Nov 25, 2009 |
Title |
GGG-37 |
Sample type |
RNA |
|
|
Source name |
CD3+ T-cells, B-CLL
|
Organism |
Homo sapiens |
Characteristics |
status: CLL cell type: CD3+ T-cells
|
Extracted molecule |
total RNA |
Extraction protocol |
Isolation of total RNA was performed with QIAGEN Rneasy columns according to the manufacturer's instructions
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA was prepared according to the standard Affymetrix protocol from 6µg total RNA
|
|
|
Hybridization protocol |
Following fragmentation, 10µg of cRNA were hybridized for 16 hr at 45°C on HG-U133A Arrays. The Arrays were washed and stained in a Affymetrix Fluidics Station 400
|
Scan protocol |
Arrays were scanned using the GeneChip Scanner G3000
|
Description |
Immunomagnetically purified CD3+ cells from peripheral blood of B-CLL
|
Data processing |
MAS5 expression values and detection calls were computed with R version 2.8.1 and the Bioconductor package affy using default parameters
|
|
|
Submission date |
Nov 23, 2009 |
Last update date |
Nov 24, 2009 |
Contact name |
Lewin Eisele |
E-mail(s) |
lewin.eisele@uni-due.de
|
Organization name |
University Hospital Essen
|
Department |
Haematology
|
Street address |
Hufelandstr. 55
|
City |
Essen |
ZIP/Postal code |
45122 |
Country |
Germany |
|
|
Platform ID |
GPL96 |
Series (1) |
GSE19147 |
CD3+ T-cells of B-cell chronic lymphocytic leukemia |
|