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Sample GSM474685 Query DataSets for GSM474685
Status Public on Nov 25, 2009
Title GJ-28
Sample type RNA
 
Source name CD3+ T-cells, B-CLL
Organism Homo sapiens
Characteristics status: CLL
cell type: CD3+ T-cells
Extracted molecule total RNA
Extraction protocol Isolation of total RNA was performed with QIAGEN Rneasy columns according to the manufacturer's instructions
Label biotin
Label protocol Biotinylated cRNA was prepared according to the standard Affymetrix protocol from 6µg total RNA
 
Hybridization protocol Following fragmentation, 10µg of cRNA were hybridized for 16 hr at 45°C on HG-U133A Arrays. The Arrays were washed and stained in a Affymetrix Fluidics Station 400
Scan protocol Arrays were scanned using the GeneChip Scanner G3000
Description Immunomagnetically purified CD3+ cells from peripheral blood of B-CLL
Data processing MAS5 expression values and detection calls were computed with R version 2.8.1 and the Bioconductor package affy using default parameters
 
Submission date Nov 23, 2009
Last update date Nov 24, 2009
Contact name Lewin Eisele
E-mail(s) lewin.eisele@uni-due.de
Organization name University Hospital Essen
Department Haematology
Street address Hufelandstr. 55
City Essen
ZIP/Postal code 45122
Country Germany
 
Platform ID GPL96
Series (1)
GSE19147 CD3+ T-cells of B-cell chronic lymphocytic leukemia

Data table header descriptions
ID_REF
VALUE MAS5
ABS_CALL

Data table
ID_REF VALUE ABS_CALL
1007_s_at 261.05 P
1053_at 163.45 P
117_at 106.15 A
121_at 869.48 P
1255_g_at 61.46 A
1294_at 749.2 P
1316_at 151.46 M
1320_at 12.38 A
1405_i_at 5897.65 P
1431_at 84.46 M
1438_at 31.11 A
1487_at 398.06 A
1494_f_at 195.09 A
1598_g_at 285.18 A
160020_at 255.82 P
1729_at 1377.83 P
177_at 60.65 A
1773_at 115.85 A
179_at 788.33 A
1861_at 177.73 P

Total number of rows: 22283

Table truncated, full table size 425 Kbytes.




Supplementary file Size Download File type/resource
GSM474685.CEL.gz 3.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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