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Sample GSM474690 Query DataSets for GSM474690
Status Public on Nov 25, 2009
Title MH-30
Sample type RNA
 
Source name CD3+ T-cells, B-CLL
Organism Homo sapiens
Characteristics status: CLL
cell type: CD3+ T-cells
Extracted molecule total RNA
Extraction protocol Isolation of total RNA was performed with QIAGEN Rneasy columns according to the manufacturer's instructions
Label biotin
Label protocol Biotinylated cRNA was prepared according to the standard Affymetrix protocol from 6µg total RNA
 
Hybridization protocol Following fragmentation, 10µg of cRNA were hybridized for 16 hr at 45°C on HG-U133A Arrays. The Arrays were washed and stained in a Affymetrix Fluidics Station 400
Scan protocol Arrays were scanned using the GeneChip Scanner G3000
Description Immunomagnetically purified CD3+ cells from peripheral blood of B-CLL
Data processing MAS5 expression values and detection calls were computed with R version 2.8.1 and the Bioconductor package affy using default parameters
 
Submission date Nov 23, 2009
Last update date Nov 24, 2009
Contact name Lewin Eisele
E-mail(s) lewin.eisele@uni-due.de
Organization name University Hospital Essen
Department Haematology
Street address Hufelandstr. 55
City Essen
ZIP/Postal code 45122
Country Germany
 
Platform ID GPL96
Series (1)
GSE19147 CD3+ T-cells of B-cell chronic lymphocytic leukemia

Data table header descriptions
ID_REF
VALUE MAS5
ABS_CALL

Data table
ID_REF VALUE ABS_CALL
1007_s_at 342.57 P
1053_at 76.61 P
117_at 128.75 P
121_at 555.26 P
1255_g_at 70.06 A
1294_at 836.3 P
1316_at 114.13 P
1320_at 5.56 A
1405_i_at 4863.45 P
1431_at 38.48 A
1438_at 17.1 A
1487_at 292.69 A
1494_f_at 177.46 A
1598_g_at 420.97 A
160020_at 266.17 M
1729_at 1552.54 P
177_at 90.43 A
1773_at 130.47 A
179_at 459.11 A
1861_at 145.65 A

Total number of rows: 22283

Table truncated, full table size 424 Kbytes.




Supplementary file Size Download File type/resource
GSM474690.CEL.gz 3.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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