|
| Status |
Public on Nov 25, 2009 |
| Title |
RG-29 |
| Sample type |
RNA |
| |
|
| Source name |
CD3+ T-cells, B-CLL
|
| Organism |
Homo sapiens |
| Characteristics |
status: CLL
cell type: CD3+ T-cells
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Isolation of total RNA was performed with QIAGEN Rneasy columns according to the manufacturer's instructions
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared according to the standard Affymetrix protocol from 6µg total RNA
|
| |
|
| Hybridization protocol |
Following fragmentation, 10µg of cRNA were hybridized for 16 hr at 45°C on HG-U133A Arrays. The Arrays were washed and stained in a Affymetrix Fluidics Station 400
|
| Scan protocol |
Arrays were scanned using the GeneChip Scanner G3000
|
| Description |
Immunomagnetically purified CD3+ cells from peripheral blood of B-CLL
|
| Data processing |
MAS5 expression values and detection calls were computed with R version 2.8.1 and the Bioconductor package affy using default parameters
|
| |
|
| Submission date |
Nov 23, 2009 |
| Last update date |
Nov 24, 2009 |
| Contact name |
Lewin Eisele |
| E-mail(s) |
lewin.eisele@uni-due.de
|
| Organization name |
University Hospital Essen
|
| Department |
Haematology
|
| Street address |
Hufelandstr. 55
|
| City |
Essen |
| ZIP/Postal code |
45122 |
| Country |
Germany |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE19147 |
CD3+ T-cells of B-cell chronic lymphocytic leukemia |
|
