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Sample GSM475560 Query DataSets for GSM475560
Status Public on Nov 26, 2009
Title B-cells
Sample type RNA
 
Source name FACS sorted B-cells
Organism Homo sapiens
Characteristics cell type: B-cells
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using the miRNeasy mini kit (Qiagen) according to the manufacturer's protocol.
Label biotin
Label protocol Samples were spiked with RNA spike control oligos (Genisphere) and labeled according to the GenisphereÒ solution using the Genisphere FlashTag™ Biotin RNA Labeling Kit.
 
Hybridization protocol Biotin labeled samples were mixed with the eukaryotic hybridization controls (Affymetrix) and hybridized to Affymetrix miRNA arrays. Arrays were subsequently washed and stained in the GeneChip fluidics station 450 (Affymetrix).
Scan protocol To assess the raw probe signal intensities, chips were scanned using the GeneChip scanner 3000 (Affymetrix).
Description miRNA expression data of B-cells
Data processing RMA normalization and probe summarization was carried out on the human specific and control probe sets only.
 
Submission date Nov 25, 2009
Last update date Nov 25, 2009
Contact name Rekin's Janky
E-mail(s) Nucleomics.Bioinformatics@vib.be
Organization name VIB
Department Nucleomics Core
Street address Herestraat 49 Box 816
City Leuven
ZIP/Postal code B-3000
Country Belgium
 
Platform ID GPL8786
Series (1)
GSE19183 miRNA profiling of FACS sorted human leukocyte cell types

Data table header descriptions
ID_REF
VALUE log2 RMA signal

Data table
ID_REF VALUE
14q-0_st 0.261475098
14qI-1_st -0.012002515
14qI-2_st 0.212257449
14qI-3_x_st 0.15231074
14qI-4_st -0.120612877
14qI-4_x_st 0.418346621
14qI-5_st 0.364570752
14qI-6_st -0.053025247
14qI-6_x_st 0.122119903
14qI-7_st 0.046054821
14qI-8_st 0.144075369
14qI-8_x_st 0.144075369
14qI-9_x_st 0.342615286
14qII-10_st 0.161341935
14qII-11_st 0.342615286
14qII-11_x_st 0.342615286
14qII-12_st -0.038109312
14qII-12_x_st 0.012883745
14qII-13_st 0.122119903
14qII-14_st 0.124718504

Total number of rows: 1959

Table truncated, full table size 54 Kbytes.




Supplementary file Size Download File type/resource
GSM475560.CEL.gz 135.2 Kb (ftp)(http) CEL
Processed data included within Sample table

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