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Sample GSM475612 Query DataSets for GSM475612
Status Public on Nov 25, 2009
Title 12.5 ng/hr leptin_4
Sample type RNA
 
Source name adult male liver, treated with 12.5 ng/hr leptin
Organism Mus musculus
Characteristics tissue: liver
agent: leptin
dose: 12.5 ng/hr
time: 12 days
Treatment protocol Animals were treated for 12 days with leptin at defined doses.
Extracted molecule total RNA
Extraction protocol Livers were extracted and flash frozen and later processed for RNA using a Trizol protocol.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description adult male mouse
4240351001_D
Data processing The data were normalised using quantile normalisation with IlluminaGUI in R
 
Submission date Nov 25, 2009
Last update date Mar 08, 2017
Contact name Kristina Hedbacker
E-mail(s) kristina.hedbaker@rockefeller.edu
Organization name Rockefeller University
Street address 1230 York Avenue
City New York
State/province NY
ZIP/Postal code 10065
Country USA
 
Platform ID GPL6885
Series (1)
GSE19185 Low dose Leptin (25 ng/hr and 12.5 ng/hr) in ob/ob mice

Data table header descriptions
ID_REF
VALUE quantile-normalised signal intensity
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1220234 392.6114 0
ILMN_2592166 236.4881 0
ILMN_3158509 958.0172 0
ILMN_1236788 319.5324 0
ILMN_2881950 714.1008 0
ILMN_2930897 246.3373 0
ILMN_2860915 3757.971 0
ILMN_2818498 1646.139 0
ILMN_2798129 434.8979 0
ILMN_1254721 2709.326 0
ILMN_1227404 972.9395 0
ILMN_2680054 1509.078 0
ILMN_1216720 214.3337 0
ILMN_2965669 294.9888 0
ILMN_2693922 835.0624 0
ILMN_3052260 441.3784 0
ILMN_3128725 406.413 0
ILMN_2835423 1048.901 0
ILMN_1213805 946.3132 0
ILMN_2655184 2534.304 0

Total number of rows: 25697

Table truncated, full table size 712 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table
Processed data are available on Series record

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