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Sample GSM475614 Query DataSets for GSM475614
Status Public on Nov 25, 2009
Title 25 ng/hr leptin_6
Sample type RNA
 
Source name adult male liver, treated with 25 ng/hr leptin
Organism Mus musculus
Characteristics tissue: liver
agent: untreated
dose: 25 ng/hr
time: 12 days
Treatment protocol Animals were treated for 12 days with leptin at defined doses.
Extracted molecule total RNA
Extraction protocol Livers were extracted and flash frozen and later processed for RNA using a Trizol protocol.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description adult male mouse
4240351001_F
Data processing The data were normalised using quantile normalisation with IlluminaGUI in R
 
Submission date Nov 25, 2009
Last update date Mar 08, 2017
Contact name Kristina Hedbacker
E-mail(s) kristina.hedbaker@rockefeller.edu
Organization name Rockefeller University
Street address 1230 York Avenue
City New York
State/province NY
ZIP/Postal code 10065
Country USA
 
Platform ID GPL6885
Series (1)
GSE19185 Low dose Leptin (25 ng/hr and 12.5 ng/hr) in ob/ob mice

Data table header descriptions
ID_REF
VALUE quantile-normalised signal intensity
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1220234 12471.77 0
ILMN_2592166 1275.826 0
ILMN_3158509 4390.796 0
ILMN_1236788 889.3671 0
ILMN_2881950 2894.283 0
ILMN_2930897 694.2252 0
ILMN_2860915 8604.338 0
ILMN_2818498 7474.907 0
ILMN_2798129 1799.938 0
ILMN_1254721 6088.933 0
ILMN_1227404 3091.77 0
ILMN_2680054 1811.288 0
ILMN_1216720 774.2604 0
ILMN_2965669 317.3221 0
ILMN_2693922 2749.464 0
ILMN_3052260 1498.357 0
ILMN_3128725 1543.823 0
ILMN_2835423 2316.656 0
ILMN_1213805 1922.957 0
ILMN_2655184 4250.515 0

Total number of rows: 25697

Table truncated, full table size 712 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table
Processed data are available on Series record

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