|
| Status |
Public on Sep 01, 2020 |
| Title |
4105_sIRI-Nx |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
sIRI-Nx
|
| Organism |
Mus musculus |
| Characteristics |
tissue: kidney
strain: NMRI
gender: Male
|
| Treatment protocol |
On day 0 mice were anasthesised with 4 mg/kg ketamine-xylazin. Unilateral IRI was performed using atraumatic vascular clip or sham operation was performed. 7 days later mice were again anasthetised and the contralateral kidney was removed or sham operation was performed. On day 8 all mice were sacrificed.
|
| Growth protocol |
animal housing: Mice were kept under standard animal-house conditions with free access to standard rodent chow and tap water ad libitum
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Mice were anticoagulated with 10 ml/kg (550 IU/mL) heparin intraperitoneally 2 min prior to cervical dislocation. The post-ischemic or sham operated left kidney was removed, snap frozen in liquid nitrogen and stored at -80°C
|
| Label |
Hy3
|
| Label protocol |
Total RNA (750 ng) from both the test and pooled reference samples were labelled with Hy3™ and Hy5™ fluorescent label, respectively, using the miRCURY LNA™ microRNA Hi-Power Labeling Kit, Hy3™/Hy5™ (Exiqon, Denmark)
|
| |
|
| Channel 2 |
| Source name |
kidney pooled reference
|
| Organism |
Mus musculus |
| Characteristics |
tissue: kidney
strain: NMRI
gender: Male
|
| Treatment protocol |
On day 0 mice were anasthesised with 4 mg/kg ketamine-xylazin. Unilateral IRI was performed using atraumatic vascular clip or sham operation was performed. 7 days later mice were again anasthetised and the contralateral kidney was removed or sham operation was performed. On day 8 all mice were sacrificed.
|
| Growth protocol |
animal housing: Mice were kept under standard animal-house conditions with free access to standard rodent chow and tap water ad libitum
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Mice were anticoagulated with 10 ml/kg (550 IU/mL) heparin intraperitoneally 2 min prior to cervical dislocation. The post-ischemic or sham operated left kidney was removed, snap frozen in liquid nitrogen and stored at -80°C
|
| Label |
Hy5
|
| Label protocol |
Total RNA (750 ng) from both the test and pooled reference samples were labelled with Hy3™ and Hy5™ fluorescent label, respectively, using the miRCURY LNA™ microRNA Hi-Power Labeling Kit, Hy3™/Hy5™ (Exiqon, Denmark)
|
| |
|
| |
| Hybridization protocol |
Hybridization wascarried out according to the miRCURY LNA™ microRNA Array Instruction manual utilizing a Tecan HS4800™ hybridization station (Tecan, Austria)
|
| Scan protocol |
Agilent G2565BA Microarray Scanner System (Agilent Technologies, Inc., USA)
|
| Description |
Biological replicate 2 of 4 from the sham IRI-Nx group, 8 days after the first intervention
|
| Data processing |
Image analysis: ImaGene® 9 (miRCURY LNA™ microRNA Array Analysis Software, Exiqon, Denmark)
Quantified signals were background corrected with Normexp method (offset value 10), for normalization the global Lowess (Locally Weighted Scatterplot Smoothing) regression algorithm was used
normalized data matrix contains the log2 normalized Hy3/Hy5 ratio
|
| |
|
| Submission date |
Aug 31, 2020 |
| Last update date |
Sep 01, 2020 |
| Contact name |
Pál Tod |
| E-mail(s) |
todpal90@gmail.com
|
| Phone |
+36205070038
|
| Organization name |
Institute of Experimental Medicine
|
| Lab |
Laboratory of Molecular Pharmacology
|
| Street address |
Szigony utca 43
|
| City |
Budapest |
| State/province |
Budapest |
| ZIP/Postal code |
H-1083 |
| Country |
Hungary |
| |
|
| Platform ID |
GPL17107 |
| Series (1) |
| GSE157221 |
miRNome changes in the mouse kidney after unilateral ischemia-reperfusion injury and delayed contralateral nephrectomy |
|
