|
Status |
Public on Feb 04, 2010 |
Title |
KO_429 |
Sample type |
RNA |
|
|
Source name |
liver KO 429
|
Organism |
Mus musculus |
Characteristics |
genotype/variation: Ugcg-deficient
|
Extracted molecule |
total RNA |
Extraction protocol |
mRNA was isolated according to Chomczynski, P. & Sacchi, N. (1987) Anal. Biochem. 162, 156–159
|
Label |
Biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
|
|
Hybridization protocol |
Hybridization (16h x 45°C) was processed according to the standard Affymetrix protocol.
|
Scan protocol |
Manufacturer's recommended protocols. Affymetrix GeneArray Scanner3000
|
Description |
Gene expression data from Ugcg-deficient mouse liver
|
Data processing |
The data were analyzed with a commercial software called JMP Genomics, version 3.2, from SAS. Gene expression profiling was performed using arrays of mouse430_2 -type from Affymetrix. A Custom CDF Version 12 with Entrez based gene definitions was used to annotate the arrays. The Raw fluorescence intensity values were normalized applying quantile normalization.
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|
|
Submission date |
Dec 04, 2009 |
Last update date |
Dec 28, 2009 |
Contact name |
Carsten Sticht |
Organization name |
University Heidelberg
|
Department |
ZMF
|
Street address |
Theodor-Kutzer-Ufer
|
City |
Mannheim |
ZIP/Postal code |
68169 |
Country |
Germany |
|
|
Platform ID |
GPL9746 |
Series (1) |
GSE19316 |
Hepatic glycosphingolipid deficiency and liver function in mice |
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