|
| Status |
Public on Feb 04, 2010 |
| Title |
WT_430 |
| Sample type |
RNA |
| |
|
| Source name |
liver WT 430
|
| Organism |
Mus musculus |
| Characteristics |
genotype/variation: wildtype
|
| Extracted molecule |
total RNA |
| Extraction protocol |
mRNA was isolated according to Chomczynski, P. & Sacchi, N. (1987) Anal. Biochem. 162, 156–159
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Hybridization (16h x 45°C) was processed according to the standard Affymetrix protocol.
|
| Scan protocol |
Manufacturer's recommended protocols. Affymetrix GeneArray Scanner3000
|
| Description |
Gene expression data from wildtype mouse liver
|
| Data processing |
The data were analyzed with a commercial software called JMP Genomics, version 3.2, from SAS. Gene expression profiling was performed using arrays of mouse430_2 -type from Affymetrix. A Custom CDF Version 12 with Entrez based gene definitions was used to annotate the arrays. The Raw fluorescence intensity values were normalized applying quantile normalization.
|
| |
|
| Submission date |
Dec 04, 2009 |
| Last update date |
Dec 28, 2009 |
| Contact name |
Carsten Sticht |
| Organization name |
University Heidelberg
|
| Department |
ZMF
|
| Street address |
Theodor-Kutzer-Ufer
|
| City |
Mannheim |
| ZIP/Postal code |
68169 |
| Country |
Germany |
| |
|
| Platform ID |
GPL9746 |
| Series (1) |
| GSE19316 |
Hepatic glycosphingolipid deficiency and liver function in mice |
|
