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Sample GSM480066 Query DataSets for GSM480066
Status Public on Dec 31, 2012
Title 5dpf_eye_rep1
Sample type RNA
 
Source name Wild type whole eye on 5dpf
Organism Danio rerio
Characteristics age: 5dpf
tissue: eye
Extracted molecule total RNA
Extraction protocol Total RNA was extracted with Qiashredder columns and the RNeasy Minikit (Qiagen, Hilden, Germany) in an RNase-free environment.
Label Biotin
Label protocol Total RNA were labeled by Affymetrix GeneChip Two-Cycle Target Labeling protocol.
 
Hybridization protocol Hybridization, washing and staining were done according to a standard procedure from Affymetrix.
Scan protocol Scanning was done according to a standard procedure from Affymetrix.
Description Eyes from 5dpf zebrafish embryo, replicate 1
Data processing The signal intensity of the microarray was normalized and summarized using the Bioconductor package, gcrma, using default setting.
 
Submission date Dec 04, 2009
Last update date Dec 31, 2012
Contact name Breandan Kennedy
E-mail(s) brendan.kennedy@ucd.ie
Organization name UCD Conway Institute, University College Dublin
Department UCD School of Biomolecular and Biomedical Science
Lab Kennedy Lab
Street address Belfield
City Dublin
ZIP/Postal code Dublin 4
Country Ireland
 
Platform ID GPL1319
Series (1)
GSE19320 Zebrafish early eye development

Data table header descriptions
ID_REF Affymetrix Probe Set ID
VALUE Log2 signal intensity normalized and summarized using gcrma with default setting

Data table
ID_REF VALUE
AFFX-BioB-3_at 5.65700585341305
AFFX-BioB-5_at 5.76711561796936
AFFX-BioB-M_at 6.95930415556227
AFFX-BioC-3_at 4.86060946082036
AFFX-BioC-5_at 6.01104317047235
AFFX-BioDn-3_at 9.98693037522072
AFFX-BioDn-5_at 9.93951850796996
AFFX-CreX-3_at 13.5357372528667
AFFX-CreX-5_at 12.6409992214308
AFFX-DapX-3_at 9.18795678390431
AFFX-DapX-5_at 3.52024695755305
AFFX-DapX-M_at 7.9837674756029
AFFX-Dr-AB076373-1_at 2.04945484659133
AFFX-Dr-AF292559-1_at 1.92302451229986
AFFX-Dr-AF292559-2_s_at 4.0411681734797
AFFX-Dr-AF292559-3_s_at 3.11690473862713
AFFX-Dr-AF292559-4_s_at 2.18786385848083
AFFX-Dr-AF292560-1_s_at 2.93174041283115
AFFX-Dr-AF298789-1_at 2.03454389661890
AFFX-Dr-AF323980-1_at 2.40603660986689

Total number of rows: 15617

Table truncated, full table size 513 Kbytes.




Supplementary file Size Download File type/resource
GSM480066_S0164F002.CEL.gz 2.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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