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Sample GSM480068 Query DataSets for GSM480068
Status Public on Dec 31, 2012
Title 3dpf_eye_rep2
Sample type RNA
 
Source name Wild type whole eye on 3dpf
Organism Danio rerio
Characteristics age: 3dpf
tissue: eye
Extracted molecule total RNA
Extraction protocol Total RNA was extracted with Qiashredder columns and the RNeasy Minikit (Qiagen, Hilden, Germany) in an RNase-free environment.
Label Biotin
Label protocol Total RNA were labeled by Affymetrix GeneChip Two-Cycle Target Labeling protocol.
 
Hybridization protocol Hybridization, washing and staining were done according to a standard procedure from Affymetrix.
Scan protocol Scanning was done according to a standard procedure from Affymetrix.
Description Eyes from 3dpf zebrafish embryo, replicate 2
Data processing The signal intensity of the microarray was normalized and summarized using the Bioconductor package, gcrma, using default setting.
 
Submission date Dec 04, 2009
Last update date Dec 31, 2012
Contact name Breandan Kennedy
E-mail(s) brendan.kennedy@ucd.ie
Organization name UCD Conway Institute, University College Dublin
Department UCD School of Biomolecular and Biomedical Science
Lab Kennedy Lab
Street address Belfield
City Dublin
ZIP/Postal code Dublin 4
Country Ireland
 
Platform ID GPL1319
Series (1)
GSE19320 Zebrafish early eye development

Data table header descriptions
ID_REF Affymetrix Probe Set ID
VALUE Log2 signal intensity normalized and summarized using gcrma with default setting

Data table
ID_REF VALUE
AFFX-BioB-3_at 5.61416291897651
AFFX-BioB-5_at 5.79466390020631
AFFX-BioB-M_at 6.72213036706307
AFFX-BioC-3_at 4.81935437381606
AFFX-BioC-5_at 5.90883480030237
AFFX-BioDn-3_at 9.94485491679646
AFFX-BioDn-5_at 9.6128378430192
AFFX-CreX-3_at 13.0819805567011
AFFX-CreX-5_at 12.1552679735774
AFFX-DapX-3_at 7.42162055432359
AFFX-DapX-5_at 2.81210034958546
AFFX-DapX-M_at 6.30514039971686
AFFX-Dr-AB076373-1_at 1.92261911152656
AFFX-Dr-AF292559-1_at 1.78433583149182
AFFX-Dr-AF292559-2_s_at 3.70064962390063
AFFX-Dr-AF292559-3_s_at 2.94749271069005
AFFX-Dr-AF292559-4_s_at 1.98491735104279
AFFX-Dr-AF292560-1_s_at 2.73250876959623
AFFX-Dr-AF298789-1_at 1.88192233071498
AFFX-Dr-AF323980-1_at 2.24861433221915

Total number of rows: 15617

Table truncated, full table size 513 Kbytes.




Supplementary file Size Download File type/resource
GSM480068_S0173F001.CEL.gz 2.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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