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Sample GSM480069 Query DataSets for GSM480069
Status Public on Dec 31, 2012
Title 5dpf_eye_rep2
Sample type RNA
 
Source name Wild type whole eye on 5dpf
Organism Danio rerio
Characteristics age: 5dpf
tissue: eye
Extracted molecule total RNA
Extraction protocol Total RNA was extracted with Qiashredder columns and the RNeasy Minikit (Qiagen, Hilden, Germany) in an RNase-free environment.
Label Biotin
Label protocol Total RNA were labeled by Affymetrix GeneChip Two-Cycle Target Labeling protocol.
 
Hybridization protocol Hybridization, washing and staining were done according to a standard procedure from Affymetrix.
Scan protocol Scanning was done according to a standard procedure from Affymetrix.
Description Eyes from 5dpf zebrafish embryo, replicate 2
Data processing The signal intensity of the microarray was normalized and summarized using the Bioconductor package, gcrma, using default setting.
 
Submission date Dec 04, 2009
Last update date Dec 31, 2012
Contact name Breandan Kennedy
E-mail(s) brendan.kennedy@ucd.ie
Organization name UCD Conway Institute, University College Dublin
Department UCD School of Biomolecular and Biomedical Science
Lab Kennedy Lab
Street address Belfield
City Dublin
ZIP/Postal code Dublin 4
Country Ireland
 
Platform ID GPL1319
Series (1)
GSE19320 Zebrafish early eye development

Data table header descriptions
ID_REF Affymetrix Probe Set ID
VALUE Log2 signal intensity normalized and summarized using gcrma with default setting

Data table
ID_REF VALUE
AFFX-BioB-3_at 6.0284659815703
AFFX-BioB-5_at 6.00498614990086
AFFX-BioB-M_at 7.79530706238885
AFFX-BioC-3_at 5.84079060451285
AFFX-BioC-5_at 6.7602732052268
AFFX-BioDn-3_at 10.6387956713575
AFFX-BioDn-5_at 10.5245199268158
AFFX-CreX-3_at 13.4708170457406
AFFX-CreX-5_at 12.8014108420039
AFFX-DapX-3_at 7.0661836467326
AFFX-DapX-5_at 2.92692393316533
AFFX-DapX-M_at 5.86041266290861
AFFX-Dr-AB076373-1_at 1.97772923889697
AFFX-Dr-AF292559-1_at 1.83662407956577
AFFX-Dr-AF292559-2_s_at 4.17548063011766
AFFX-Dr-AF292559-3_s_at 3.07937189320016
AFFX-Dr-AF292559-4_s_at 2.12537853071979
AFFX-Dr-AF292560-1_s_at 2.8962921380414
AFFX-Dr-AF298789-1_at 1.93984582276954
AFFX-Dr-AF323980-1_at 2.32755023069107

Total number of rows: 15617

Table truncated, full table size 513 Kbytes.




Supplementary file Size Download File type/resource
GSM480069_S0173F002.CEL.gz 2.1 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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