|
| Status |
Public on Dec 31, 2012 |
| Title |
5dpf_eye_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Wild type whole eye on 5dpf
|
| Organism |
Danio rerio |
| Characteristics |
age: 5dpf
tissue: eye
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with Qiashredder columns and the RNeasy Minikit (Qiagen, Hilden, Germany) in an RNase-free environment.
|
| Label |
Biotin
|
| Label protocol |
Total RNA were labeled by Affymetrix GeneChip Two-Cycle Target Labeling protocol.
|
| |
|
| Hybridization protocol |
Hybridization, washing and staining were done according to a standard procedure from Affymetrix.
|
| Scan protocol |
Scanning was done according to a standard procedure from Affymetrix.
|
| Description |
Eyes from 5dpf zebrafish embryo, replicate 2
|
| Data processing |
The signal intensity of the microarray was normalized and summarized using the Bioconductor package, gcrma, using default setting.
|
| |
|
| Submission date |
Dec 04, 2009 |
| Last update date |
Dec 31, 2012 |
| Contact name |
Breandan Kennedy |
| E-mail(s) |
brendan.kennedy@ucd.ie
|
| Organization name |
UCD Conway Institute, University College Dublin
|
| Department |
UCD School of Biomolecular and Biomedical Science
|
| Lab |
Kennedy Lab
|
| Street address |
Belfield
|
| City |
Dublin |
| ZIP/Postal code |
Dublin 4 |
| Country |
Ireland |
| |
|
| Platform ID |
GPL1319 |
| Series (1) |
| GSE19320 |
Zebrafish early eye development |
|
