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Sample GSM480070 Query DataSets for GSM480070
Status Public on Dec 31, 2012
Title 3dpf_eye_rep3
Sample type RNA
 
Source name Wild type whole eye on 3dpf
Organism Danio rerio
Characteristics age: 3dpf
tissue: eye
Extracted molecule total RNA
Extraction protocol Total RNA was extracted with Qiashredder columns and the RNeasy Minikit (Qiagen, Hilden, Germany) in an RNase-free environment.
Label Biotin
Label protocol Total RNA were labeled by Affymetrix GeneChip Two-Cycle Target Labeling protocol.
 
Hybridization protocol Hybridization, washing and staining were done according to a standard procedure from Affymetrix.
Scan protocol Scanning was done according to a standard procedure from Affymetrix.
Description Eyes from 3dpf zebrafish embryo, replicate 3
Data processing The signal intensity of the microarray was normalized and summarized using the Bioconductor package, gcrma, using default setting.
 
Submission date Dec 04, 2009
Last update date Dec 31, 2012
Contact name Breandan Kennedy
E-mail(s) brendan.kennedy@ucd.ie
Organization name UCD Conway Institute, University College Dublin
Department UCD School of Biomolecular and Biomedical Science
Lab Kennedy Lab
Street address Belfield
City Dublin
ZIP/Postal code Dublin 4
Country Ireland
 
Platform ID GPL1319
Series (1)
GSE19320 Zebrafish early eye development

Data table header descriptions
ID_REF Affymetrix Probe Set ID
VALUE Log2 signal intensity normalized and summarized using gcrma with default setting

Data table
ID_REF VALUE
AFFX-BioB-3_at 5.64981932023751
AFFX-BioB-5_at 6.14496752703515
AFFX-BioB-M_at 7.0813795359726
AFFX-BioC-3_at 4.82182311643284
AFFX-BioC-5_at 5.95943707839397
AFFX-BioDn-3_at 10.2850395114378
AFFX-BioDn-5_at 9.94922667706529
AFFX-CreX-3_at 13.2867918736273
AFFX-CreX-5_at 12.5175477704827
AFFX-DapX-3_at 7.11487327147521
AFFX-DapX-5_at 2.87571192239777
AFFX-DapX-M_at 5.99661906770974
AFFX-Dr-AB076373-1_at 1.9535005691801
AFFX-Dr-AF292559-1_at 1.81408912640199
AFFX-Dr-AF292559-2_s_at 3.86467214567068
AFFX-Dr-AF292559-3_s_at 2.98915836014662
AFFX-Dr-AF292559-4_s_at 2.05101118281067
AFFX-Dr-AF292560-1_s_at 2.80234552212923
AFFX-Dr-AF298789-1_at 1.90697870863103
AFFX-Dr-AF323980-1_at 2.27893957710824

Total number of rows: 15617

Table truncated, full table size 513 Kbytes.




Supplementary file Size Download File type/resource
GSM480070_S0173F005.CEL.gz 2.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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