|
Status |
Public on Dec 31, 2012 |
Title |
3dpf_eye_rep3 |
Sample type |
RNA |
|
|
Source name |
Wild type whole eye on 3dpf
|
Organism |
Danio rerio |
Characteristics |
age: 3dpf tissue: eye
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted with Qiashredder columns and the RNeasy Minikit (Qiagen, Hilden, Germany) in an RNase-free environment.
|
Label |
Biotin
|
Label protocol |
Total RNA were labeled by Affymetrix GeneChip Two-Cycle Target Labeling protocol.
|
|
|
Hybridization protocol |
Hybridization, washing and staining were done according to a standard procedure from Affymetrix.
|
Scan protocol |
Scanning was done according to a standard procedure from Affymetrix.
|
Description |
Eyes from 3dpf zebrafish embryo, replicate 3
|
Data processing |
The signal intensity of the microarray was normalized and summarized using the Bioconductor package, gcrma, using default setting.
|
|
|
Submission date |
Dec 04, 2009 |
Last update date |
Dec 31, 2012 |
Contact name |
Breandan Kennedy |
E-mail(s) |
brendan.kennedy@ucd.ie
|
Organization name |
UCD Conway Institute, University College Dublin
|
Department |
UCD School of Biomolecular and Biomedical Science
|
Lab |
Kennedy Lab
|
Street address |
Belfield
|
City |
Dublin |
ZIP/Postal code |
Dublin 4 |
Country |
Ireland |
|
|
Platform ID |
GPL1319 |
Series (1) |
GSE19320 |
Zebrafish early eye development |
|