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Sample GSM480071 Query DataSets for GSM480071
Status Public on Dec 31, 2012
Title 5dpf_eye_rep3
Sample type RNA
 
Source name Wild type whole eye on 5dpf
Organism Danio rerio
Characteristics age: 5dpf
tissue: eye
Extracted molecule total RNA
Extraction protocol Total RNA was extracted with Qiashredder columns and the RNeasy Minikit (Qiagen, Hilden, Germany) in an RNase-free environment.
Label Biotin
Label protocol Total RNA were labeled by Affymetrix GeneChip Two-Cycle Target Labeling protocol.
 
Hybridization protocol Hybridization, washing and staining were done according to a standard procedure from Affymetrix.
Scan protocol Scanning was done according to a standard procedure from Affymetrix.
Description Eyes from 5dpf zebrafish embryo, replicate 3
Data processing The signal intensity of the microarray was normalized and summarized using the Bioconductor package, gcrma, using default setting.
 
Submission date Dec 04, 2009
Last update date Dec 31, 2012
Contact name Breandan Kennedy
E-mail(s) brendan.kennedy@ucd.ie
Organization name UCD Conway Institute, University College Dublin
Department UCD School of Biomolecular and Biomedical Science
Lab Kennedy Lab
Street address Belfield
City Dublin
ZIP/Postal code Dublin 4
Country Ireland
 
Platform ID GPL1319
Series (1)
GSE19320 Zebrafish early eye development

Data table header descriptions
ID_REF Affymetrix Probe Set ID
VALUE Log2 signal intensity normalized and summarized using gcrma with default setting

Data table
ID_REF VALUE
AFFX-BioB-3_at 6.6518870810981
AFFX-BioB-5_at 7.13907742211632
AFFX-BioB-M_at 7.99869158447302
AFFX-BioC-3_at 6.10345059571233
AFFX-BioC-5_at 7.10005843545384
AFFX-BioDn-3_at 10.8678606900694
AFFX-BioDn-5_at 10.7108082995982
AFFX-CreX-3_at 13.5669930895112
AFFX-CreX-5_at 12.9506815625529
AFFX-DapX-3_at 7.526455984114
AFFX-DapX-5_at 2.98192578530057
AFFX-DapX-M_at 6.29958233036291
AFFX-Dr-AB076373-1_at 2.02792588572438
AFFX-Dr-AF292559-1_at 1.88104441961296
AFFX-Dr-AF292559-2_s_at 4.61774543823975
AFFX-Dr-AF292559-3_s_at 3.24341869349257
AFFX-Dr-AF292559-4_s_at 2.14250280932325
AFFX-Dr-AF292560-1_s_at 2.99786493906818
AFFX-Dr-AF298789-1_at 1.98811276362272
AFFX-Dr-AF323980-1_at 2.48299348141677

Total number of rows: 15617

Table truncated, full table size 513 Kbytes.




Supplementary file Size Download File type/resource
GSM480071_S0173F006.CEL.gz 2.1 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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