|
| Status |
Public on May 01, 2013 |
| Title |
CNS PNET 03R |
| Sample type |
RNA |
| |
|
| Source name |
Tumor material, snap frozen during surgery
|
| Organism |
Homo sapiens |
| Characteristics |
tumour type: CNS PNET
|
| Growth protocol |
Tumor material was removed during surgery then snap frozen
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the mirVana miRNA isolation kit (Ambion)
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared from 5 mg RNA using the Affymetrix HT IVT labeling method (one cycle)
|
| |
|
| Hybridization protocol |
Labeled RNA was hybridized for 16 hours at 45 oC on the U133 plus 2 array. GeneChips were washed and stained in the fluidics station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip scanner 8000 with autoloader
|
| Description |
no additional information
|
| Data processing |
Raw data was analysed using Expression console. RMA was used to generate data for analysis. Processed data was analysed in Spotfire DecisionSite for Functional Genomics (TIBCO Software Inc.) for hierarchical clustering and priniciple component analysis. BRB Array Tools Software ver 3.7 (http://linus.nci.nih.gov/BRB-ArrayTools.html) was used for t-tests.
|
| |
|
| Submission date |
Dec 09, 2009 |
| Last update date |
May 01, 2013 |
| Contact name |
Hazel Anne Rogers |
| E-mail(s) |
hazel.rogers@nottingham.ac.uk
|
| Organization name |
University of Nottingham
|
| Department |
Clinical Sciences
|
| Lab |
D30
|
| Street address |
Medical School
|
| City |
Nottingham |
| ZIP/Postal code |
NG7 2UH |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE19404 |
Gene expression analysis of primitive neuroectodermal tumors |
|
