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Sample GSM482083 Query DataSets for GSM482083
Status Public on May 01, 2013
Title CNS PNET 07
Sample type RNA
Source name Tumor material, snap frozen during surgery
Organism Homo sapiens
Characteristics tumour type: CNS PNET
Growth protocol Tumor material was removed during surgery then snap frozen
Extracted molecule total RNA
Extraction protocol RNA was extracted using the mirVana miRNA isolation kit (Ambion)
Label biotin
Label protocol Biotinylated cRNA was prepared from 5 mg RNA using the Affymetrix HT IVT labeling method (one cycle)
Hybridization protocol Labeled RNA was hybridized for 16 hours at 45 oC on the U133 plus 2 array. GeneChips were washed and stained in the fluidics station 450.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip scanner 8000 with autoloader
Description no additional information
Data processing Raw data was analysed using Expression console. RMA was used to generate data for analysis. Processed data was analysed in Spotfire DecisionSite for Functional Genomics (TIBCO Software Inc.) for hierarchical clustering and priniciple component analysis. BRB Array Tools Software ver 3.7 ( was used for t-tests.
Submission date Dec 09, 2009
Last update date May 01, 2013
Contact name Hazel Anne Rogers
Organization name University of Nottingham
Department Clinical Sciences
Lab D30
Street address Medical School
City Nottingham
ZIP/Postal code NG7 2UH
Country United Kingdom
Platform ID GPL570
Series (1)
GSE19404 Gene expression analysis of primitive neuroectodermal tumors

Data table header descriptions
VALUE RMA signal intensity

Data table
AFFX-BioB-5_at 7.684035
AFFX-BioB-M_at 8.239716
AFFX-BioB-3_at 7.553521
AFFX-BioC-5_at 9.127602
AFFX-BioC-3_at 9.438353
AFFX-BioDn-5_at 10.11317
AFFX-BioDn-3_at 11.47684
AFFX-CreX-5_at 12.69505
AFFX-CreX-3_at 13.08067
AFFX-DapX-5_at 3.313107
AFFX-DapX-M_at 2.782974
AFFX-DapX-3_at 3.159063
AFFX-LysX-5_at 2.863631
AFFX-LysX-M_at 2.988811
AFFX-LysX-3_at 3.467564
AFFX-PheX-5_at 2.882541
AFFX-PheX-M_at 3.007496
AFFX-PheX-3_at 4.890134
AFFX-ThrX-5_at 3.444766
AFFX-ThrX-M_at 2.591152

Total number of rows: 54675

Table truncated, full table size 1052 Kbytes.

Supplementary file Size Download File type/resource
GSM482083.CEL.gz 4.4 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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