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Sample GSM482097 Query DataSets for GSM482097
Status Public on May 01, 2013
Title MB 08
Sample type RNA
 
Source name Tumor material, snap frozen during surgery
Organism Homo sapiens
Characteristics tumour type: Medulloblastoma
Growth protocol Tumor material was removed during surgery then snap frozen
Extracted molecule total RNA
Extraction protocol RNA was extracted using the mirVana miRNA isolation kit (Ambion)
Label biotin
Label protocol Biotinylated cRNA was prepared from 5 mg RNA using the Affymetrix HT IVT labeling method (one cycle)
 
Hybridization protocol Labeled RNA was hybridized for 16 hours at 45 oC on the U133 plus 2 array. GeneChips were washed and stained in the fluidics station 450.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip scanner 8000 with autoloader
Description no additional information
Data processing Raw data was analysed using Expression console. RMA was used to generate data for analysis. Processed data was analysed in Spotfire DecisionSite for Functional Genomics (TIBCO Software Inc.) for hierarchical clustering and priniciple component analysis. BRB Array Tools Software ver 3.7 (http://linus.nci.nih.gov/BRB-ArrayTools.html) was used for t-tests.
 
Submission date Dec 09, 2009
Last update date May 01, 2013
Contact name Hazel Anne Rogers
E-mail(s) hazel.rogers@nottingham.ac.uk
Organization name University of Nottingham
Department Clinical Sciences
Lab D30
Street address Medical School
City Nottingham
ZIP/Postal code NG7 2UH
Country United Kingdom
 
Platform ID GPL570
Series (1)
GSE19404 Gene expression analysis of primitive neuroectodermal tumors

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 7.54055
AFFX-BioB-M_at 8.001227
AFFX-BioB-3_at 7.323886
AFFX-BioC-5_at 8.748583
AFFX-BioC-3_at 9.170791
AFFX-BioDn-5_at 9.786595
AFFX-BioDn-3_at 11.02724
AFFX-CreX-5_at 12.6581
AFFX-CreX-3_at 13.01678
AFFX-DapX-5_at 7.996546
AFFX-DapX-M_at 8.567034
AFFX-DapX-3_at 9.117703
AFFX-LysX-5_at 4.49252
AFFX-LysX-M_at 5.227199
AFFX-LysX-3_at 6.080595
AFFX-PheX-5_at 5.621313
AFFX-PheX-M_at 5.591317
AFFX-PheX-3_at 6.288884
AFFX-ThrX-5_at 6.071684
AFFX-ThrX-M_at 6.255145

Total number of rows: 54675

Table truncated, full table size 1051 Kbytes.




Supplementary file Size Download File type/resource
GSM482097.CEL.gz 4.4 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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