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Sample GSM484667 Query DataSets for GSM484667
Status Public on Mar 21, 2010
Title MS, replicate 2
Sample type RNA
 
Source name FACS sorted mammary cell subpopulation
Organism Mus musculus
Characteristics gender: female
cell subpopulation: MS
mouse pool (biological replicate): 2
hybridization batch: 1
beadchip barcode: 4481850187
beadchip section: B
Extracted molecule total RNA
Extraction protocol RNA from sorted cell populations was prepared with the RNeasy Micro kit (Qiagen). RNA quality was ascertained using the Agilent Bioanalyser 2100 with the NanoChip protocol.
Label Cy3
Label protocol We labeled up to 500 ng with the Total Prep RNA amplification kit (Ambion).
 
Hybridization protocol Labeled cRNA (1.5 μg) was prepared for hybridization to Illumina mouseWG-6 V2 (R1) BeadChips.
Scan protocol After washing, we coupled the chips with Cy3 and scanned them in an Illumina BeadArray Reader.
Description no additional information
Data processing Un-normalized summary probe profiles were output from BeadStudio and analysed using the limma packages of the Bioconductor open-source software project (http://www.bioconductor.org). The raw intensities were normalized using the neqc function with offset 16.
 
Submission date Dec 13, 2009
Last update date Mar 21, 2010
Contact name Jane E Visvader
E-mail(s) visvader@wehi.edu.au
Phone (+61 3) 93452494
Fax (+61 3) 93470852
Organization name The Walter and Eliza Hall Institute of Medical Research
Department Victorian Breast Cancer Research Consortium
Lab Lindeman/Visvader Lab
Street address 1G Royal Parade
City Parkville
State/province Victoria
ZIP/Postal code 3052
Country Australia
 
Platform ID GPL6887
Series (1)
GSE19446 Gene expression profiles of normal mouse mammary cell subpopulations FACS sorted based on expression of biomarkers

Data table header descriptions
ID_REF
VALUE limma-software normalized log2-intensity

Data table
ID_REF VALUE
ILMN_2417611 4.721880721
ILMN_2762289 4.751117858
ILMN_2896528 12.36287455
ILMN_2721178 10.39420823
ILMN_2458837 4.792122785
ILMN_3033922 10.89435999
ILMN_3092673 12.63646133
ILMN_1230777 11.95495816
ILMN_1246069 11.44419032
ILMN_1232042 4.772943298
ILMN_1243193 10.0135419
ILMN_2524361 4.975059941
ILMN_1242440 5.008167244
ILMN_1233188 8.31633863
ILMN_2543688 10.67959382
ILMN_1259789 6.558252664
ILMN_2816356 6.599412727
ILMN_1224596 7.811830305
ILMN_1233643 4.539190783
ILMN_2808939 10.62581167

Total number of rows: 45281

Table truncated, full table size 1100 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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